Purification, structural elucidation and antitumor activity of a novel mannogalactoglucan from the fruiting bodies of Lentinus edodes
Purification, structural elucidation and antitumor activity of a novel mannogalactoglucan from the fruiting bodies of Lentinus edodes
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DOI：
10.1016/j.fitote.2012.12.008
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发表时间：
2013-01-01
期刊：
影响因子：
3.4
通讯作者：
Zhou, Yifa
中科院分区：
文献类型：
--
作者：
Jeff, Iteku B.;Li, Shanshan;Zhou, Yifa
采用热水提取、乙醇沉淀、阴离子交换层析、凝胶渗透层析等方法从香菇担子果皮中分离得到一种甘露半乳葡聚糖，命名为LE-MGG。通过高效液相色谱(HPLC)、高效凝胶渗透色谱(HPGPC)、甲基化分析、高碘酸氧化-Smith降解、红外光谱(IR)和核磁共振波谱(包括二维核磁共振)对其结构进行了表征。高效液相色谱分析表明，LE-MGG含有甘露糖-半乳糖-葡萄糖的摩尔比为10：18：72。GPC和HPGPC分析表明，LE-MGG为均一组分(d=1.34)，其相对分子质量为18 kDa。化学和光谱研究表明，LE-MGG由(1-gt；6)-、(1-gt；4)-和(1-gt；3)连接的β-D-吡喃葡萄糖基、(1-gt；6)连接的α-D-半乳糖基残基、(1-gt；3，6)-和(1-gt；2，4)连接的α-D-甘露糖基残基和末端残基组成。细胞毒性实验表明，LE-MGG对S-180细胞具有较高的抗肿瘤活性，且呈剂量依赖关系，而对人肝癌细胞株HCT116和HT29的细胞毒性较低。我们的研究还表明，LE-MGG对移植于昆明小鼠的S180实体瘤细胞具有抗肿瘤活性。这一发现表明，甘露半乳葡聚糖应作为潜在的抗肿瘤药物进行开发，并可能作为一种天然的抗肿瘤药物应用。(C)2012爱思唯尔B.V.保留所有权利。
A mannogalactoglucan, named LE-MGG, was isolated from the basidiocarps of Lentinus edodes by hot water-extraction, ethanol precipitation anion exchange chromatography, and further purified by gel-permeation chromatography (GPC). Its structural features were investigated by high performance liquid chromatography (HPLC), high performance gel-permeation chromatography (HPGPC), methylation analysis, periodate oxidation-Smith degradation, and by IR and NMR spectroscopy, including two-dimensional (2D) NMR. HPLC analysis revealed that LE-MGG contained mannose-galactose-glucose in the molar ratio of 10:18:72. GPC and HPGPC showed that LE-MGG was a homogeneous fraction (d = 1.34) and its molecular weight was estimated to be 18 kDa. Chemical and spectroscopic studies indicated that LE-MGG consists of (1 -> 6)-, (1 -> 4)- and (1 -> 3)-linked beta-D-glucopyranosyl residues, (1 -> 6)-linked alpha-D-galactopyranosyl residues, (1 -> 3,6)- and (1 -> 2,4)-linked alpha-D-mannopyranosyl residues and terminal residues of beta-D-glucopyranosyl. Cytotoxicity assay showed that LE-MGG presented higher antitumor activities against S-180 cell with a dose-dependent manner, and exhibited lower cytotoxicity to carcinoma HCT-116 and HT-29 cells. Our studies showed also that LE-MGG presented antitumor bioactivities on Sarcoma 180 solid tumor cell implanted in Kunming mice. This finding suggests that mannogalactoglucan should be explored as potential antitumor agents and could be potentially applied as a natural antitumor drug. (c) 2012 Elsevier B.V. All rights reserved.