A modified TMV-based vector facilitates the expression of longer foreign epitopes in tobacco
A modified TMV-based vector facilitates the expression of longer foreign epitopes in tobacco
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DOI：
10.1016/j.vaccine.2005.09.060
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发表时间：
2006-01-12
期刊：
影响因子：
5.5
通讯作者：
Xu, ZK
中科院分区：
文献类型：
--
作者：
Jiang, LB;Li, QL;Xu, ZK
以烟草花叶病毒（TMV）侵染的烟草突变体为基础，构建了一种新的烟草花叶病毒载体，该载体在病毒外壳蛋白（CP）亚基上缺失了4 ~ 6个c端氨基酸残基。新载体在豚鼠和猪对口蹄疫病毒（FMDV）的感染性、病毒颗粒的产量和更重要的F11的保护活性方面与原基于tmv的载体非常相似，均表达了烟草中血清0型口蹄疫病毒Vp1的表位肽F11 (p（142) -A(152)）。此外，新载体编码的外源肽长度的表达能力大大提高，成功表达了含有FMDV VP1两个融合表位F14 （R-200-L-213）和F11的肽F25，而原载体在烟草中表达失败。虽然动物实验表明，这种表达的F25在免疫中的效率不如F11，可能是由于在两个融合的表位之间缺乏间隔臂，但新的基于tmv的载体可能满足表达不同疫苗和其他药物的更长的外源肽的要求。(c) 2005年Elsevier Ltd出版
Based upon a mutant isolated from tobacco infected with a recombinant tobacco mosaic vir-us (TMV), a new TMV-based vector was developed in which four to six C-terminal amino acid residues were deleted from the viral coat protein (CP) subunit. The new vector was quite similar to the original TMV-based vector, which all expressed a well characterized epitope peptide F11 (p(142) -A(152)) of Vp1 from foot-and-mouth disease virus (FMDV) serotype 0 in tobacco, in the infectivity, yield of the virus particles and more importantly protective activity of F11 in guinea pigs and swine against the FMDV. Furthermore, the capacity of the length of foreign peptide encoded by this new vector was much improved to successfully express a peptide F25 containing two fused epitopes F14 (R-200-L-213) and F11 of FMDV VP1, which was failed using the original vector in tobacco. Although animal assays indicated that such expressed F25 was not as efficient as F11 in the immunity, possibly due to lack of a spacer arm between the two fused epitopes, the new TMV-based vector may meet the requirement of expressing longer foreign peptides for different vaccines and other medicines. (c) 2005 Published by Elsevier Ltd.