Transcriptomics-based Analysis of the Response of Sugar Content in Litchi Pulp to Foliar Calcium Fertilizer Treatment
Transcriptomics-based Analysis of the Response of Sugar Content in Litchi Pulp to Foliar Calcium Fertilizer Treatment
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DOI：
10.21273/jashs05258-22
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发表时间：
2023-01
影响因子：
1.9
通讯作者：
Junjie Peng;Jingjia Du;Tiantian Chen;Xian Shui;Haizhi Liao;Xiaokai Lin;Kaibing Zhou
中科院分区：
文献类型：
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作者：
Junjie Peng;Jingjia Du;Tiantian Chen;Xian Shui;Haizhi Liao;Xiaokai Lin;Kaibing Zhou
以10株16年生荔枝为试验材料，研究叶面钙肥对‘妃子笑’荔枝果肉糖度的影响。 2020年和2021年的试验在花后35天（DAA）开始，处理为叶面喷施0.3%CaCl2水溶液，对照为叶面喷施水。测量了2020年和2021年果肉中的糖含量、蔗糖代谢酶和ATP依赖性磷酸果糖激酶（PFK）活性。对2020年35、63和69 DAA（完全成熟阶段）处理和对照果肉的RNA样本进行转录组测序（RNA-seq），选择10个基因通过实时聚合酶链进行确认2020年和2021年进行了PCR反应。完全成熟时，钙处理组可溶性糖含量极显着或显着高于对照组。 63 DAA后，钙处理组的净蔗糖代谢酶活性显着高于对照组。此外，在完全成熟时，钙处理组的蔗糖合酶裂解活性显着高于对照组，而PFK活性显着低于对照组。从转录组数据中筛选出54个糖酵解途径（EMP）高表达基因，包括己糖激酶、PFK、丙酮酸激酶基因； 2020 年 69 DAA 时，与对照组相比，治疗组中有 87% 的这些基因下调。RNA-seq 和实时 PCR 结果之间的线性回归在 2020 年 (r = 0.9292) 和 2021 年 (r = 0.8889) 显着。当果实完全成熟时，钙处理通过增加蔗糖合酶裂解活性来增加净蔗糖代谢酶活性，促进还原糖积累，并下调EMP中磷酸果糖激酶基因的表达，促进糖积累。
Ten 16-year-old trees were used as test materials to investigate the effect of foliar calcium fertilizer on the sugar content of ‘Feizixiao’ litchi (Litchi chinensis Sonn.) pulp. The experiment began 35 days after anthesis (DAA) in 2020 and 2021, and the treatment was a foliar spray application of 0.3% CaCl2 aqueous solution, whereas the control was a foliar spray application of water. The sugar content, sucrose-metabolizing enzymes, and ATP-dependent phosphofructokinase (PFK) activities in pulp were measured in 2020 and 2021. Transcriptome sequencing (RNA-seq) was performed on RNA samples from treatment and control fruit pulps at 35, 63, and 69 DAA (full mature stage) in 2020, and 10 genes were chosen for confirmation by real-time polymerase chain reaction (PCR) in 2020 and 2021. At full maturity, the soluble sugar content in the calcium-treated group was extremely significantly or significantly higher than that in the control group. After 63 DAA, the net sucrose-metabolizing enzyme activity in the calcium-treated group was significantly higher than that in the control group. Furthermore, at full maturity, the calcium-treated group had significantly higher sucrose synthase cleavage activity and significantly lower PFK activity than the control group. Fifty-four highly expressed genes in the glycolytic pathway (EMP) were screened from transcriptome data, including hexokinase, PFK, and pyruvate kinase genes; 87% of these genes were downregulated in the treatment group compared with the control group at 69 DAA in 2020. The linear regression between RNA-seq and real-time PCR results was significant in 2020 (r = 0.9292) and 2021 (r = 0.8889). When the fruit is fully ripe, calcium treatment increases net sucrose-metabolizing enzyme activity by increasing sucrose synthase cleavage activity, promoting the accumulation of reducing sugars, and it downregulates phosphofructokinase gene expression in EMP, promoting sugar accumulation.