CHARACTERIZATION OF THE BINDING OF [3H]MUSCIMOL, A POTENT γ‐AMINOBUTYRIC ACID AGONIST, TO RAT BRAIN SYNAPTOSOMAL MEMBRANES USING A FILTRATION ASSAY
CHARACTERIZATION OF THE BINDING OF [3H]MUSCIMOL, A POTENT γ‐AMINOBUTYRIC ACID AGONIST, TO RAT BRAIN SYNAPTOSOMAL MEMBRANES USING A FILTRATION ASSAY
复制标题
使用过滤测定法表征 [3H]muscimol（一种有效的 γ-氨基丁酸激动剂）与大鼠脑突触体膜的结合
DOI：
10.1111/j.1471-4159.1979.tb04553.x
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发表时间：
1979
影响因子：
4.7
通讯作者：
E. Risley
中科院分区：
文献类型：
--
作者：
M. Williams;E. Risley
用滤膜法分离膜结合配体，研究了一种有效的GABA激动剂[~3H]麝香酚与大鼠脑粗面突触膜的结合。特异性结合是饱和的，并发生在KD5 5和30 nm的两个结合部位。结合是不依赖于Na+的，冷冻和Triton处理都增强了结合。与突触GABA受体结合的区域和亚细胞分布研究和药理学特征与特异性的[~3H]Muscimol结合一致。
Abstract— The binding of [3H]muscimol, a potent GABA agonist, to crude synaptic membranes prepared from rat brain was studied using a filtration method to isolate membrane‐bound ligand. Specific binding was found to be saturable and occurred to two binding sites of Kd5 5 and 30 nm. Binding was Na+‐independent and enhanced by both freezing and Triton treatment. Regional and subcellular distribution studies and pharmacological characterization of specific [3H]muscimol binding are consistent with binding to the synaptic GABA receptor.