Molecular cloning, characterization, and chromosome mapping of reptilian estrogen receptors.
Molecular cloning, characterization, and chromosome mapping of reptilian estrogen receptors.
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DOI：
10.1210/en.2010-0356
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发表时间：
2010-12
期刊：
影响因子：
4.8
通讯作者：
Y. Katsu;K. Matsubara;S. Kohno;Y. Matsuda;M. Toriba;Kaori Oka;L. Guillette;Y. Ohta;T. Iguchi
中科院分区：
文献类型：
--
作者：
Y. Katsu;K. Matsubara;S. Kohno;Y. Matsuda;M. Toriba;Kaori Oka;L. Guillette;Y. Ohta;T. Iguchi
在许多脊椎动物中，类固醇激素对于关键发育阶段的卵巢分化以及促进成年雌性生殖系统的生长和分化是必不可少的。虽然已经在哺乳动物和一些鱼类、两栖动物和鸟类中进行了广泛的研究，但在爬行动物中对性类固醇激素(雌激素)作用的分子机制研究得很少。在此，我们分离了编码雌激素受体α(Esr1)和雌激素受体β(Esr2)的基因，研究了两种蛇--冲绳哈布(Protobothrops flavviridis，Viperidae)和日本四纹大鼠蛇(elaphe four rivirgata，Colubridae)的激素受体和配体的相互作用。利用哺乳动物细胞瞬时转染实验，检测了爬行动物(冲绳哈布、日本四纹鼠蛇、美洲扬子鳄和佛罗里达红腹淡水龟)ESR1和ESR2的转录活性。所有ESR蛋白都通过含有雌激素反应元件的启动子表现出雌激素依赖的转录激活；然而，对不同雌激素的反应是不同的。此外，我们还确定了蛇类固醇激素受体基因的染色体位置。ESR1和ESR2基因分别定位于1号染色体的短臂和长臂，而雄激素受体定位于两种蛇的一对小染色体上。这些数据提供了基本的工具，为未来研究受体-配体相互作用和蛇的类固醇内分泌学提供了基础，也扩大了我们对性类固醇激素受体进化的了解。
In many vertebrates, steroid hormones are essential for ovarian differentiation during a critical developmental stage as well as promoting the growth and differentiation of the adult female reproductive system. Although studies have been extensively conducted in mammals and a few fish, amphibians, and bird species, the molecular mechanisms of sex steroid hormone (estrogens) action have been poorly examined in reptiles. Here, we evaluate hormone receptor and ligand interactions in two species of snake, the Okinawa habu (Protobothrops flavoviridis, Viperidae) and the Japanese four-striped rat snake (Elaphe quadrivirgata, Colubridae) after the isolation of cDNAs encoding estrogen receptor α (ESR1) and estrogen receptor β (ESR2). Using a transient transfection assay with mammalian cells, the transcriptional activity of reptilian (Okinawa habu, Japanese four-striped rat snake, American alligator, and Florida red-belly freshwater turtle) ESR1 and ESR2 was examined. All ESR proteins displayed estrogen-dependent activation of transcription via an estrogen-response element-containing promoter; however, the responsiveness to various estrogens was different. Further, we determined the chromosomal locations of the snake steroid hormone receptor genes. ESR1 and ESR2 genes were localized to the short and long arms of chromosome 1, respectively, whereas androgen receptor was localized to a pair of microchromosomes in the two snake species examined. These data provide basic tools that allow future studies examining receptor-ligand interactions and steroid endocrinology in snakes and also expands our knowledge of sex steroid hormone receptor evolution.