Microfluidic Diffusion Viscometer for Rapid Analysis of Complex Solutions
Microfluidic Diffusion Viscometer for Rapid Analysis of Complex Solutions
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DOI：
10.1021/acs.analchem.5b02930
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发表时间：
2016-04-05
影响因子：
7.4
通讯作者：
Knowles, Tuomas P. J.
中科院分区：
文献类型：
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作者：
Arosio, Paolo;Hu, Kevin;Knowles, Tuomas P. J.
复杂溶液的粘度是与材料、生物和生物技术科学中的大量应用相关的重要物理性质。在这里，我们展示了一种微流控技术来测量溶液的粘度以下的平流和扩散的示踪剂颗粒在稳态流动。我们用标准的水-甘油混合物验证我们的方法，然后我们应用这种微流体扩散粘度计来测量高浓度蛋白质溶液的粘度以及粗细胞裂解物的粘度。我们的方法表现出一系列有吸引力的功能，包括分析时间的顺序为秒和几μ L的样品的消耗，以及容易集成的微流体粘度计在其他仪器平台或模块化微流体设备的可能性。这些特性使得微流体扩散粘度计在生物技术和医疗保健科学中的自动化过程中成为一种有吸引力的方法，其中需要以有限的样品消耗量进行快速测量。
The viscosity of complex solutions is a physical property of central relevance for a large number of applications in material, biological, and biotechnological sciences. Here we demonstrate a microfluidic technology to measure the viscosity of solutions by following the advection and diffusion of tracer particles under steady-state flow. We validate our method with standard water-glycerol mixtures, and then we apply this microfluidic diffusion viscometer to measure the viscosity of protein solutions at high concentrations as well as of a crude cell lysate. Our approach exhibits a series of attractive features, including analysis time on the order of seconds and the consumption of a few mu L of sample, as well as the possibility to readily integrate the microfluidic viscometer in other instrument platforms or modular microfluidic devices. These characteristics make microfluidic diffusion viscometry an attractive approach in automated processes in biotechnology and health-care sciences where fast measurements with limited amount of sample consumption are required.