Antioxidant Activities and Anti-Cancer Cell Proliferation Properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry Cultivars.
Antioxidant Activities and Anti-Cancer Cell Proliferation Properties of Natsuhaze (Vaccinium oldhamii Miq.), Shashanbo (V. bracteatum Thunb.) and Blueberry Cultivars.
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DOI：
10.3390/plants2010057
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发表时间：
2013-02-15
期刊：
影响因子：
--
通讯作者：
Yukizaki C
中科院分区：
文献类型：
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作者：
Tsuda H;Kunitake H;Kawasaki-Takaki R;Nishiyama K;Yamasaki M;Komatsu H;Yukizaki C
蓝莓中含有丰富的抗氧化剂，虽然关于水果的生物活性化合物的研究很多，但野生越橘因其丰富多样的化学成分而引起人们的注意。本研究旨在研究日本蓝莓栽培品种和野生种的生物活性成分。在越桔属植物的5种提取物中，有Natsuhaze(Vaccdium oldhamii MIQ.)在体外对HL-60人白血病细胞生长的抑制作用最强。虽然所有乙醇提取物对HL-60细胞都有生长抑制作用，但作用程度因物种而异。水提物可诱导HL-60细胞出现凋亡小体和核小体DNA片段化。在供试的提取物中，纳豆的总多酚含量最高，具有最强的抗氧化活性，但其花色苷含量与兔眼蓝莓相近。结果表明，总多酚具有较高的抗氧化活性和对HL-60人白血病细胞的生长抑制作用。
Antioxidants are abundant in blueberries, and while there are many studies concerning the bioactive compound of fruit, it is only recently that the wild Vaccinium species has attracted attention for their diverse and abundant chemical components. The aim of this study was to investigate the bioactive compounds of blueberry cultivars and wild species found in Japan. Among the five extracts of the Vaccinium species, Natsuhaze (Vaccinium oldhamii Miq.) was found to be the most effective at inhibiting the growth of HL-60 human leukemia cells in vitro. Although all ethanol extracts showed a growth inhibitory effect on HL-60 cells, the degree of the effects differed among the species. The extract of Natsuhaze induced apoptotic bodies and nucleosomal DNA fragmentation in the HL-60 cells. Of the extracts tested, that of Natsuhaze contained the largest amount of total polyphenols and showed the greatest antioxidant activity, but the anthocyanin content of Natsuhaze was similar to that of rabbiteye blueberry (V. virgatum Ait.). The results showed that total polyphenols contributed to the high antioxidant activity and growth inhibitory effect on HL-60 human leukemia cells of Natsuhaze extract.