Melatonin inhibits DNA synthesis in MCF-7 human breast cancer cells in vitro
Melatonin inhibits DNA synthesis in MCF-7 human breast cancer cells in vitro
复制标题
DOI：
10.1016/0024-3205(96)00249-4
复制
发表时间：
1996-05-24
期刊：
影响因子：
6.1
通讯作者：
SanchezBarcelo, EJ
中科院分区：
文献类型：
--
作者：
Cos, S;Fernandez, F;SanchezBarcelo, EJ
本工作的目的是研究生理剂量的褪黑素(1 Nm)是否对MCF-7人乳腺癌细胞的DNA合成有影响。将指数生长期的MCF-7细胞与胸腺嘧啶核苷(2 MM)孵育24 h，以阻断有丝分裂并同步细胞分裂周期。用流式细胞仪检测同步性，结果显示，从过量的胸腺嘧啶核苷释放后，82.3%的细胞处于G(1)期。在有丝分裂停止后的0、3、6、9、12、15或24小时，用[H-3]脱氧胸腺嘧啶核苷([H-3]dThy)或[H-3]dThy+褪黑素冲击这些同步细胞。这些同步化的MCF-7细胞仅对褪黑素作用1h，在6h或9h时显著抑制[H-3]dThy的掺入，从有丝分裂阻断释放后，DNA前体掺入最高，S期细胞数最多。我们的结论是，至少在一定程度上，褪黑素对MCF-7细胞的抗增殖作用可能是通过减少DNA合成来实现的。
The aim of the present work was to study whether physiological doses of melatonin (1nM) modified DNA synthesis in MCF-7 human breast cancer cells. Exponentially growing MCF-7 cells were incubated for 24 h with thymidine (2mM) for blocking mitosis and synchronizing the cell division cycle. Synchronization was assessed by a flow cytometry study which showed that after release from excess thymidine, 82.3% of the cells were in phase G(1). Lots of these synchronized cells were pulsed for Ih with [H-3]deoxythymidine ([H-3]dThy) or [H-3]dThy + melatonin, at 0, 3, 6, 9, 12, 15 or 24 h from the release of the mitotic arrest. The exposition of these synchronized MCF-7 cells to melatonin for only Ih, significantly inhibited [H-3]dThy incorporation when it was at 6 or 9 h. after release from mitotic block at a time when DNA precursor incorporation was the highest and the number of cells in S phase was maximum. We conclude that, at least in part, melatonin antiproliferative effects on MCF-7 cells could be mediated by a reduction of DNA synthesis.