An electrochemical dual-signaling aptasensor for the ultrasensitive detection of insulin
An electrochemical dual-signaling aptasensor for the ultrasensitive detection of insulin
复制标题
用于超灵敏检测胰岛素的电化学双信号适体传感器
DOI：
10.1016/j.ab.2019.02.032
复制
发表时间：
2019
影响因子：
2.9
通讯作者：
Wu Huiyun
中科院分区：
文献类型：
--
作者：
Zhao Yana;Xu Youyou;Zhang Manman;Xiang Juan;Deng Chunyan;Wu Huiyun
胰岛素在生理糖代谢中起着核心作用，并与糖尿病和相关疾病有关。本文报道了一种高灵敏度、高特异度的双信号电化学适体传感器。以亚甲基蓝(MB)修饰的胰岛素结合适体(IBA)为“信号关闭”探针，(DNA2)/二茂铁(FC)共修饰的金纳米颗粒(DNA2Fc@GNPs)为“信号开启”探针，与连接体mDNA组装成DNA2Fc@GNPs/mDNA/MB-IBA修饰的金电极作为传感界面，以MB和FC的电流响应作为信号指示剂。不出所料，胰岛素与DNA2Fc@GNPs/mDNA/MB-IBA/Au电极孵育后，MB和FC的电流响应分别降低和增加。在此基础上，成功地实现了胰岛素的检测，在10~10 nm范围内线性关系良好，最低检测浓度为0.1 pm。通过对血清中胰岛素浓度的测定，证明该适体传感器具有较高的特异性和准确性。此外，双信令通过其自参考能力有助于更准确和更可重复性的检测。该适配子传感器具有简单、快速、灵敏度高、特异性强、准确度高等优点，对提高胰岛素相关疾病的诊断水平具有重要意义。
Insulin plays a central role in physiological glycolmetabolism and is associated with diabetes and related diseases. In this work, a dual-signaling electrochemical aptasensor for insulin detection with high sensitivity and specificity has been reported. Methylene blue (MB)-modified insulin-binding aptamer (IBA) as “signal-off” probe, and (DNA2)/Ferrocene (Fc) co-modified gold nanoparticles (DNA2Fc@GNPs) as the “signal-on” probe were integrated with linker mDNA to fabricate the DNA2Fc@GNPs/mDNA/MB-IBA modified Au electrode as the sensing interface, and the current responses of MB and Fc were used as signal indicators. As expected, the incubation of insulin with DNA2Fc@GNPs/mDNA/MB-IBA/Au electrode resulted in the current responses of MB and Fc decreased and increased, respectively. Based on this strategy, the detection of insulin was successfully achieved, and a linear range from 10 pM to 10 nM with the detectable lowest concentration of 0.1 pM was obtained. By measuring the insulin concentrations in serum samples, this proposed aptasensor has been proven to be of high specificity and accuracy. Moreover, the dual-signaling is useful for the more accurate and reproducible detection through their self-referencing capability. This aptasensor possesses such advantages as simplicity, rapid responses, high sensitivity, specificity and accuracy, which is significant for improving the diagnosis of insulin-related diseases.