Interaction of neuron-specific K+-Cl- cotransporter, KCC2, with brain-type creatine kinase
Interaction of neuron-specific K+-Cl- cotransporter, KCC2, with brain-type creatine kinase
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DOI：
10.1016/s0014-5793(04)00328-x
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发表时间：
2004-04-23
期刊：
影响因子：
3.5
通讯作者：
Fukuda, A
中科院分区：
文献类型：
--
作者：
Inoue, K;Ueno, S;Fukuda, A
γ-氨基丁酸是成人中枢神经系统内的一种主要抑制性神经递质，由于细胞内Cl-浓度升高，也已知其在早期发育阶段具有兴奋性。这种功能变化主要归因于K+-Cl-协同转运蛋白KCC 2，其表达在神经元中受到发育调节。然而，关于KCC 2功能的细胞内调节的详细信息很少。在这里，我们确定KCC 2和脑型肌酸激酶之间的相互作用，通过酵母双杂交筛选。这种相互作用，这也是在培养的细胞和脑提取物中检测到的，可能有助于KCC 2介导的调节Cl-稳态。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
gamma-Aminobutyric acid, a major inhibitory neurotransmitter within the adult central nervous system, is also known to be excitatory at early developmental stages due to the elevated intracellular Cl- concentration. This functional change is primarily attributable to a K+-Cl- cotransporter, KCC2, the expression of which is developmentally regulated in neurons. However, little detail information is available concerning the intracellular regulation of KCC2 function. Here, we identify an interaction between KCC2 and brain-type creatine kinase by means of yeast two-hybrid screening. This interaction, which was also detected in cultured cells and brain extracts, might contribute to KCC2-mediated modulation of Cl- homeostasis. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.