Anesthesia can cause sustained hyperglycemia in C57/BL6J mice
Anesthesia can cause sustained hyperglycemia in C57/BL6J mice
复制标题
DOI：
10.1017/s0952523805225105
复制
发表时间：
2005-09-01
影响因子：
1.9
通讯作者：
Barlow, R
中科院分区：
文献类型：
--
作者：
Brown, ET;Umino, Y;Barlow, R
麻醉对C57/BL 6 J小鼠血糖的影响在通常用于用视网膜电图（ERG）记录测试视网膜敏感性的条件下进行评估。我们评估了四种麻醉剂的作用：戊巴比妥（50 mg/kg）、硫喷妥钠（100 mg/kg）、阿维丁（240 mg/kg）和氯胺酮/甲苯噻嗪（100 mg/kg），以生理盐水作为对照。在腹腔注射前和注射后15分钟和60分钟，我们测量了尾静脉血中的血糖（BG）水平。注射后15分钟，所有四种麻醉剂和生理盐水均升高BG，氯胺酮/甲苯噻嗪和阿维丁的作用明显大于戊巴比妥、硫喷妥钠和生理盐水。只有氯胺酮/甲苯噻嗪和阿维丁的作用在整个测试期间持续存在。注射氯胺酮/甲苯噻嗪后60分钟，BG仍升高至400 +/- 42 mg/dl，较注射前水平增加167%。注射阿维丁后60分钟，BG为288 +/- 10 mg/dl，比注射前水平增加59%。注射戊巴比妥、硫喷妥钠或生理盐水后60分钟，未检测到BG持续升高。由于BG可影响ERG的振幅，因此应谨慎使用氯胺酮/甲苯噻嗪或阿维丁。麻醉的选择在糖尿病和代谢研究中也很重要，因为血糖的变化可能会影响生理过程。
Effects of anesthesia on the blood glucose of C57/BL6J mice were evaluated under conditions commonly used for testing retinal sensitivity with electroretinographic (ERG) recordings. We evaluated the effects of four anesthetics: nembutal (50 mg/kg), pentothal (100 mg/kg), avertin (240 mg/kg), and ketamine/xylazine (100 mg/kg) using saline as control. We measured blood glucose (BG) levels from tail vein blood before and 15 and 60 min following intraperitoneal injections. Fifteen minutes postinjection, all four anesthetics and saline elevated BG with ketamine/xylazine and avertin having substantially greater effects than nembutal, pentothal, and saline. Only the effects of ketamine/xylazine and avertin persisted throughout the test period. Sixty minutes after injecting ketamine/xylazine BG remained elevated at 400 +/- 42 mg/dl, a 167% increase over preinjection levels. Sixty minutes after injecting avertin BG was 288 +/- 10 mg/dl, a 59% increase over preinjection levels. No sustained elevation in BG was detected 60 min following injection of nembutal, pentothal, or saline. Because BG can affect the amplitude of the ERG, caution should be exercised in the use of ketamine/xylazine or avertin. The choice of anesthesia may also be important in diabetes and metabolism research where changes in blood glucose could impact physiological processes.