Prenatal water deprivation alters brain angiotensin system and dipsogenic changes in the offspring.
Prenatal water deprivation alters brain angiotensin system and dipsogenic changes in the offspring.
复制标题
产前缺水会改变后代的大脑血管紧张素系统和体质变化
DOI：
10.1016/j.brainres.2011.01.031
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发表时间：
2011-03-25
期刊：
影响因子：
2.9
通讯作者：
Xu Z
中科院分区：
文献类型：
--
作者：
Zhang H;Fan Y;Xia F;Geng C;Mao C;Jiang S;He R;Zhang L;Xu Z
中枢肾素-血管紧张素系统（RAS）在调节体液平衡中发挥着重要作用。本研究确定了母体脱水对后代大脑中血管紧张素原、血管紧张素 II 受体亚型表达水平和促反应的影响。怀孕的老鼠在妊娠后期被剥夺水份。测定脑血管紧张素原、血管紧张素II受体和促反应的表达。母亲缺水会显着降低胎儿的身体和大脑重量以及身体和尾巴的长度。胎儿血浆钠、渗透压和血细胞比容增加。胎儿大脑中 AT1R 和 AT2R 蛋白丰度均显着增加，与 AT1aR 和 AT2R mRNA 水平增加相关。此外，血管紧张素原 mRNA 增加。在成年后代中，产前脱水导致雄性和雌性前脑中 AT1R 蛋白和 AT1aR mRNA 以及血管紧张素原 mRNA 显着增加。相比之下，AT2R mRNA 和蛋白质仅在男性中增加。产前脱水导致雄性后代脑室内血管紧张素 II 诱导的水摄入量显着增加，但雌性后代则不然。结果提供了新的信息，即产前缺水会诱导大脑 RAS 和 Ang II 受体表达模式的重新编程，并以性别依赖性方式改变后代中枢 Ang II 介导的动力反应。
Central renin–angiotensin system (RAS) plays an important role in regulating body fluid balance. The present study determined the effect of maternal dehydration on brain expression levels of angiotensinogen, angiotensin II receptor subtypes, and dipsogenic responses in offspring. Pregnant rats were deprived of water during late gestation. Expressions of brain angiotensinogen, angiotensin II receptors, and dipsogenic responses were determined. Maternal water deprivation significantly decreased fetal body and brain weight, and body and tail length. Fetal plasma sodium, osmolality, and hematocrit were increased. Both AT1R and AT2R protein abundance was significantly increased in the fetal brain, associating with increased mRNA levels of AT1aR and AT2R. Additionally, angiotensinogen mRNA was increased. In adult offspring, prenatal dehydration resulted in significant increases in AT1R protein and AT1aR mRNA, as well as angiotensinogen mRNA in the forebrain in both males and females. In contrast, AT2R mRNA and protein were increased only in males. Prenatal dehydration resulted in a significant increase in intracerebroventricular angiotensin II-induced water intake in male, but not female, offspring. The results provided new information that antenatal water deprivation induces a reprogramming of brain RAS and Ang II receptor expression patterns and alters the central Ang II-mediated dipsogenic response in offspring in a sex-dependent manner.