Identification of drought-induced microRNAs in rice
Identification of drought-induced microRNAs in rice
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水稻中干旱诱导的 microRNA 的鉴定。
DOI：
10.1016/j.bbrc.2007.01.022
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发表时间：
2007-03-09
影响因子：
3.1
通讯作者：
Jin, Youxin
中科院分区：
文献类型：
--
作者：
Zhao, Botao;Liang, Ruqiang;Jin, Youxin
MicroRNAs(MiRNAs)是一大类新的非编码小RNA。到目前为止，已在植物中鉴定出数百个microRNAs。NlmicroRNAs通过靶向mRNAs的切割或抑制翻译，在转录后基因调控中发挥重要作用。为了更好地了解microRNA的功能，我们使用了一个寡核苷酸芯片来监测干旱胁迫下水稻(Oryza Sativa)的microRNA表达谱。鉴定了两个干旱诱导的microRNAs。此外，miR-169G被证实是miR-169家族中唯一受干旱诱导的成员，并且miR-169G在根中的诱导作用比在地上部更显著。序列分析显示，在miR-169G的上游存在两个最近的DRE(脱水反应元件)，表明miR-169G的表达可能直接受到CBF/DREBS的调控。(C)2007 Elsevier Inc.保留所有权利。
MicroRNAs (miRNAs) are a large new class of small non-coding RNAs. To date, hundreds of microRNAs have been identified in plants. NlicroRNAs play important roles in post-transcriptional gene regulation by targeting mRNAs for cleavage or repressing translation. To better understand microRNA function, we have used an oligonucleotide microarray to monitor rice (Oryza sativa) microRNA expression profile under drought stress. Two drought-induced microRNAs were identified. Furthermore, miR-169g was confirmed as the only member induced by drought among the miR-169 family and the induction of miR-169g was more prominent in roots than in shoots. Sequence analysis revealed occurrence of two proximate DREs (dehydration-responsive element) in the upstream of the MIR-169g, suggesting that miR-169g expression may be regulated directly by CBF/DREBs. (c) 2007 Elsevier Inc. All rights reserved.