Native electrospray mass spectrometry approaches to probe the interaction between zinc and an anti-angiogenic peptide from histidine-rich glycoprotein.
Native electrospray mass spectrometry approaches to probe the interaction between zinc and an anti-angiogenic peptide from histidine-rich glycoprotein.
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DOI：
10.1038/s41598-018-26924-1
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发表时间：
2018-06-05
影响因子：
4.6
通讯作者：
Blindauer CA
中科院分区：
文献类型：
--
作者：
Martin EM;Kondrat FDL;Stewart AJ;Scrivens JH;Sadler PJ;Blindauer CA
锌通过与富含组氨酸的糖蛋白(HRR)结合来调节其生物学功能。结合行波离子迁移质谱(TWIM-MS)研究了一种模拟HRR的35个氨基酸的生物活性多肽HRGP330的锌离子结合特性。自然质谱学证实了锌与HRGP330结合；然而，添加锌离子后1H核磁共振谱的展宽排除了获得结构信息的可能性。采用TWIM-MS的补充方法表明，在锌离子存在的情况下，HRGP330具有更紧密的结构。自上而下的MS/MS数据支持金属结合诱导的构象变化，因为观察到与锌结合的HRGP330的碎片较少。碰撞诱导解离(CID)和电子转移解离/质子转移反应(ETD/PTR)实验表明，该多肽的N端和C端均有锌离子结合的片段，表明该区域存在多个结合部位。质谱学和核磁共振方法的结合为锌和这种富含组氨酸的多肽之间的高度动态相互作用提供了新的见解。
Zinc modulates the biological function of histidine-rich glycoprotein (HRG) through binding to its His-rich region (HRR). The Zn2+-binding properties of a 35 amino-acid biologically-active peptide mimic of the HRR, HRGP330, were investigated using dissociative mass spectrometry approaches in addition to travelling-wave ion mobility mass spectrometry (TWIM-MS). Native mass spectrometry confirmed zinc binding to HRGP330; however, broadening of the 1H NMR resonances upon addition of Zn2+ ions precluded the attainment of structural information. A complementary approach employing TWIM-MS indicated that HRGP330 has a more compact structure in the presence of Zn2+ ions. Top-down MS/MS data supported a metal-binding-induced conformational change, as fewer fragments were observed for Zn2+-bound HRGP330. Zn2+-bound fragments of both N-terminal and C-terminal ends of the peptide were identified from collision-induced dissociation (CID) and electron transfer dissociation/proton transfer reaction (ETD/PTR) experiments, suggesting that multiple binding sites exist within this region of HRG. The combination of mass spectrometry and NMR approaches provides new insight into the highly dynamic interaction between zinc and this His-rich peptide.
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影响因子：
4.8
作者：
Fredenburgh, James C.;Leslie, Beverly A.;Weitz, Jeffrey I.
通讯作者：
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影响因子：
2
作者：
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影响因子：
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作者：
GUTHANS, SL;MORGAN, WT
通讯作者：
MORGAN, WT
DOI：
10.1007/s13361-016-1395-z
发表时间：
2016-07-01
影响因子：
3.2
作者：
Asakawa, Daiki;De Pauw, Edwin
通讯作者：
De Pauw, Edwin
影响因子：
11.2
作者：
Dixelius, J;Olsson, AK;Claesson-Welsh, L
通讯作者：
Claesson-Welsh, L