Renal and urinary proteomics: Current applications and challenges
Renal and urinary proteomics: Current applications and challenges
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DOI：
10.1002/pmic.200401012
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发表时间：
2005-03-01
期刊：
影响因子：
3.4
通讯作者：
Malasit, P
中科院分区：
文献类型：
--
作者：
Thongboonkerd, V;Malasit, P
近年来，蛋白质组学已广泛应用于包括肾脏病在内的医学各个领域。目前肾脏和尿液蛋白质组学的应用是更好地了解肾脏生理学，探索疾病机制的复杂性，并确定新的生物标志物和新的治疗靶点。本文就蛋白质组学如何应用于肾脏病学以及实验数据如何与生理学、功能意义和临床应用联系起来提供了一些实例和观点。在某些情况下，蛋白质组学分析可用于从表达研究中获得的一组候选物中产生新的假设。新的假设，然后可以解决迅速通过传统的分子生物学方法，如通过鉴定的改变肾弹性蛋白-弹性蛋白酶系统在糖尿病肾病和改变在肾激肽释放酶-激肽释放酶途径在缺氧诱导的高血压。总结了蛋白质组学在肾脏研究中的优势和局限性。需要优化分析方案，以克服当前的局限性。蛋白质组学在肾脏病学中的应用将更加富有成果和成功。
During the past few years, proteomics has been extensively applied to various fields of medicine including nephrology. Current applications of renal and urinary proteomics are to better understand renal physiology, to explore the complexity of disease mechanisms, and to identify novel biomarkers and new therapeutic targets. This review provides some examples and perspectives of how proteomics can be applied to nephrology and how experimental data can be linked to physiology, functional significance and clinical applications. In some instances, proteomic analysis can be utilized to generate a new hypothesis from a set of candidates that are obtained from expression studies. The new hypothesis can then be addressed rapidly by conventional molecular biology methods, as demonstrated by identification of an altered renal elastin-elastase system in diabetic nephropathy and alterations in the renal kallikrein-kallistatin pathway in hypoxia-induced hypertension. The strengths and limitations of proteomics in renal research are summarized. Optimization of analytical protocols is required to overcome current limitations. Applications of proteomics to nephrology will then be more fruitful and successful.