Horizontal acquisition of multiple mitochondrial genes from a parasitic plant followed by gene conversion with host mitochondrial genes.
Horizontal acquisition of multiple mitochondrial genes from a parasitic plant followed by gene conversion with host mitochondrial genes.
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DOI：
10.1186/1741-7007-8-150
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发表时间：
2010-12-22
期刊：
影响因子：
5.4
通讯作者：
Palmer JD
中科院分区：
文献类型：
--
作者：
Mower JP;Stefanović S;Hao W;Gummow JS;Jain K;Ahmed D;Palmer JD
水平基因转移（HGT）在植物线粒体基因组中相对常见，但转移的机制、程度和后果仍不清楚。以往的研究结果表明，寄生植物往往参与无论是转移供体或受体，表明寄生虫和宿主之间的直接接触，促进植物之间的遗传转移。为了揭示植物间HGT的机制细节，研究了两个群体之间转移的程度和进化命运：寄生属菟丝子和车前草属物种的一个小分支。线粒体基因的广泛聚合酶链反应（PCR）调查显示，至少有三个基因（atp 1，atp 6和matR）最近从菟丝子转移到车前。定量PCR分析表明，这三个基因有一个线粒体的位置在一个物种线车前检查。序列进化的模式表明，这些外源基因降解成假基因后不久，转移和逆转录（RT）-PCR分析表明，没有可检测的转录。在atp 1基因的天然拷贝和外来拷贝之间检测到三例基因转换。车前属内的三个外源基因的相同的系统发育分布和胞苷在RNA编辑的祖先位置的保留表明，这些基因可能是通过一个单一的，DNA介导的转移事件获得的。然而，从两个三个物种中的受体车前分支的多个个体的采样揭示了复杂和令人困惑的系统发育差异和模式的所有三个外来基因的序列分歧。这项研究报告了迄今为止最好的证据，即多个线粒体基因可以通过一个单一的HGT事件转移，并且转移是通过严格的DNA水平的中间体发生的。外来和本地线粒体拷贝之间的基因转换的发现表明，转移的基因可能是在产生线粒体遗传多样性的进化上重要的。最后，每个谱系内的复杂关系的转移基因意味着一个令人惊讶的复杂的历史，这些基因在车前收购后，通过HGT和这个历史可能涉及一些额外的转移（包括细胞内转移），基因复制，差异损失和突变率的变化。解开这段历史可能需要对车前属的多个线粒体和核基因组进行测序。参见评论：http://www.biomedcentral.com/1741-7007/8/147。
Horizontal gene transfer (HGT) is relatively common in plant mitochondrial genomes but the mechanisms, extent and consequences of transfer remain largely unknown. Previous results indicate that parasitic plants are often involved as either transfer donors or recipients, suggesting that direct contact between parasite and host facilitates genetic transfer among plants. In order to uncover the mechanistic details of plant-to-plant HGT, the extent and evolutionary fate of transfer was investigated between two groups: the parasitic genus Cuscuta and a small clade of Plantago species. A broad polymerase chain reaction (PCR) survey of mitochondrial genes revealed that at least three genes (atp1, atp6 and matR) were recently transferred from Cuscuta to Plantago. Quantitative PCR assays show that these three genes have a mitochondrial location in the one species line of Plantago examined. Patterns of sequence evolution suggest that these foreign genes degraded into pseudogenes shortly after transfer and reverse transcription (RT)-PCR analyses demonstrate that none are detectably transcribed. Three cases of gene conversion were detected between native and foreign copies of the atp1 gene. The identical phylogenetic distribution of the three foreign genes within Plantago and the retention of cytidines at ancestral positions of RNA editing indicate that these genes were probably acquired via a single, DNA-mediated transfer event. However, samplings of multiple individuals from two of the three species in the recipient Plantago clade revealed complex and perplexing phylogenetic discrepancies and patterns of sequence divergence for all three of the foreign genes. This study reports the best evidence to date that multiple mitochondrial genes can be transferred via a single HGT event and that transfer occurred via a strictly DNA-level intermediate. The discovery of gene conversion between co-resident foreign and native mitochondrial copies suggests that transferred genes may be evolutionarily important in generating mitochondrial genetic diversity. Finally, the complex relationships within each lineage of transferred genes imply a surprisingly complicated history of these genes in Plantago subsequent to their acquisition via HGT and this history probably involves some combination of additional transfers (including intracellular transfer), gene duplication, differential loss and mutation-rate variation. Unravelling this history will probably require sequencing multiple mitochondrial and nuclear genomes from Plantago. See Commentary: http://www.biomedcentral.com/1741-7007/8/147.
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影响因子：
3.4
作者：
Barkman TJ;McNeal JR;Lim SH;Coat G;Croom HB;Young ND;Depamphilis CW
通讯作者：
Depamphilis CW
影响因子：
10.7
作者：
Goremykin, Vadim V.;Salamini, Francesco;Viola, Roberto
通讯作者：
Viola, Roberto
影响因子：
56.9
作者：
Davis, CC;Wurdack, KJ
通讯作者：
Wurdack, KJ
DOI：
10.1073/pnas.042694899
发表时间：
2002-07-23
影响因子：
11.1
作者：
Adams, KL;Qiu, YL;Palmer, JD
通讯作者：
Palmer, JD
影响因子：
10.7
作者：
Alverson, Andrew J.;Wei, XiaoXin;Palmer, Jeffrey D.
通讯作者：
Palmer, Jeffrey D.