Morphometric changes induced by amino acid supplementation in skeletal and cardiac muscles of old mice
Morphometric changes induced by amino acid supplementation in skeletal and cardiac muscles of old mice
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DOI：
10.1016/j.amjcard.2008.02.078
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发表时间：
2008-06-02
影响因子：
2.8
通讯作者：
Bianchi, Rossella
中科院分区：
文献类型：
--
作者：
Corsetti, Giovanni;Pasini, Evasio;Bianchi, Rossella
衰老与肌肉和心脏纤维的进行性结构紊乱、功能能力下降以及疾病和死亡率增加有关。衰老的特征还在于蛋白质合成紊乱，细胞器功能受损，特别是在线粒体中。氨基酸的可用性是哺乳动物整体代谢的关键因素，外源性补充氨基酸混合物（AAm）可能是改善老年人生活质量、避免营养不良和肌肉萎缩的有效治疗策略。我们研究了形态定量的影响，长期补充AAm的线粒体和肌节（通过电子显微镜）和胶原基质沉积（通过组织学技术）在年轻和老年小鼠的骨骼肌和心肌。我们的数据显示，老年动物的两块肌肉中线粒体较少，纤维化严重。长期补充AAm增加了老年大鼠骨骼肌和心脏线粒体和肌节的数量和体积，并减少了纤维化。这些结果表明，AAm恢复肌肉形态参数，并可能改善这些器官的机械性能。(c)2008年爱思唯尔公司All rights reserved.
Aging is associated with progressive structural disorganization of muscular and cardiac fibers, decreasing functional capacity, and increased rates of disease and death. Aging is also characterized by disturbances in protein synthesis with impaired cellular organelle functions, particularly in the mitochondria. The availability of amino acids is a key factor for the overall metabolism of mammals and exogenous supplements of amino acid mixtures (AAm) could be a valid therapeutic strategy to improve quality of life, avoiding malnutrition and, muscle wasting in the elderly. We investigated the morphoquantitative effects of long-term AAm supplementation on the mitochondria and sarcomeres (by electron microscope) and on collagen matrix deposition (by histologic techniques) in both skeletal and cardiac muscles of young and aged mice. Our data showed that old animals have fewer mitochondria and massive fibrosis in both muscles. Long-term AAm supplementation increased the number and volume of mitochondria and sarcomeres and decreased fibrosis in both skeletal muscle and hearts in old rats. These findings indicate that AAm restored muscular morphologic parameters and probably improved the mechanical performance of these organs. (c) 2008 Elsevier Inc. All rights reserved.