Characterization of resilin-like proteins with tunable mechanical properties.
Characterization of resilin-like proteins with tunable mechanical properties.
复制标题
具有可调机械性能的节肢弹性蛋白样蛋白的表征。
DOI：
10.1016/j.jmbbm.2018.11.015
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发表时间：
2019
影响因子：
3.9
通讯作者：
Liu,JulieC
中科院分区：
文献类型：
--
作者：
Su,RenayS-C;Gill,EmilyE;Kim,Yeji;Liu,JulieC
树脂蛋白是昆虫表皮中丰富的弹性蛋白。其卓越的性能，包括高弹性和高效的能量存储，激发了其在组织工程和药物递送应用中的潜在用途。我们的实验室先前已经开发了重组蛋白质的基础上的弹性蛋白样序列来自冈比亚按蚊，并证明了他们的承诺，作为一个支架的软骨和血管工程。在这项工作中，我们描述了一个更彻底的调查交联的节枝弹性蛋白类水凝胶的物理性质。节枝弹性蛋白样蛋白在生理条件下快速形成交联水凝胶。我们还表明，这些节枝弹性蛋白样水凝胶的机械性能可以简单地通过改变蛋白质浓度或交联剂的化学计量比交联位点调制。交联的节枝弹性蛋白样水凝胶是亲水性的，并且在溶胀时具有高含水量。此外，这些水凝胶表现出适度的弹性值，这是与普通合成橡胶。冷冻扫描电子显微镜显示，在16重量%的交联的节枝弹性蛋白样水凝胶具有蜂窝状结构。因此，这些研究表明，重组节枝弹性蛋白样蛋白由于其可调的物理性质，在各种各样的应用中，如组织工程和药物递送中使用的潜力。
Resilin is an elastomeric protein abundant in insect cuticle. Its exceptional properties, which include high resilience and efficient energy storage, motivate its potential use in tissue engineering and drug delivery applications. Our lab has previously developed recombinant proteins based on the resilin-like sequence derived fromAnopheles gambiaeand demonstrated their promise as a scaffold for cartilage and vascular engineering. In this work, we describe a more thorough investigation of the physical properties of crosslinked resilin-like hydrogels. The resilin-like proteins rapidly form crosslinked hydrogels in physiological conditions. We also show that the mechanical properties of these resilin-like hydrogels can be modulated simply by varying the protein concentration or the stoichiometric ratio of crosslinker to crosslinking sites. Crosslinked resilin-like hydrogels were hydrophilic and had a high water content when swollen. In addition, these hydrogels exhibited moderate resilience values, which were comparable to those of common synthetic rubbers. Cryo-scanning electron microscopy showed that the crosslinked resilin-like hydrogels at 16 wt% featured a honeycomb-like structure. These studies thus demonstrate the potential to use recombinant resilin-like proteins in a wide variety of applications such as tissue engineering and drug delivery due to their tunable physical properties.