UNUSUALLY LARGE TELOMERIC REPEATS IN THE YEAST CANDIDA-ALBICANS
UNUSUALLY LARGE TELOMERIC REPEATS IN THE YEAST CANDIDA-ALBICANS
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DOI：
10.1128/mcb.13.1.551
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发表时间：
1993-01-01
影响因子：
5.3
通讯作者：
HICKS, JB
中科院分区：
文献类型：
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作者：
MCEACHERN, MJ;HICKS, JB
我们已经确定了白色念珠菌的端粒序列，并发现它们是由一个23 bp序列的串联拷贝组成的。通过克隆原生端粒末端和“愈合”末端的表征和克隆，我们证明这些重复序列足以作为端粒发挥作用。从许多白色念珠菌菌株中测序的23-bp重复序列的所有拷贝都是相同的。相比之下，相邻的亚端粒序列在菌株之间和WO-1菌株内都是可变的。在WO-1菌株中，端粒的长度取决于生长温度，并且在较高温度下显着更长。端粒生长伴随着端粒片段上23-bp重复序列数量的增加。这些结果表明，要么端粒酶维持的端粒在结构上可能比以前想象的更复杂，要么念珠菌端粒是通过端粒酶独立的机制维持的。
We have identified sequences at the telomeres of the yeast Candida albicans and have found that they are composed of tandem copies of a 23-bp sequence. Through the cloning of native telomeric ends and the characterization and cloning of a ''healed'' end, we demonstrate that these repeated sequences are sufficient to function as a telomere. All copies of the 23-bp repeat that have been sequenced from a number of C albicans strains are identical. In contrast, adjacent subtelomeric sequences are variable both between strains and within the WO-1 strain. In the WO-1 strain, the lengths of the telomeres are dependent upon growth temperature and are substantially longer at higher temperatures. Telomere growth is accompanied by increases in the number of the 23-bp repeats present on the telomeric fragments. These results suggest that either telomerase-maintained telomeres can be more complex in structure than was previously imagined or that Candida telomeres are maintained via a telomerase-independent mechanism.