Effect of matrine on HeLa cell adhesion and migration
Effect of matrine on HeLa cell adhesion and migration
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DOI：
10.1016/j.ejphar.2007.01.073
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发表时间：
2007-06-01
影响因子：
5
通讯作者：
Wei, Lei
中科院分区：
文献类型：
--
作者：
Zhang, Lijun;Wang, Tingting;Wei, Lei
本研究旨在探讨苦参碱在癌细胞转移中的作用，并探讨苦参碱抑制癌细胞转移的可能机制。因此，我们首先研究了血管扩张剂刺激磷酸化蛋白（VASP）磷酸化和camp依赖性蛋白激酶（PKA）活性在细胞脱离和再附着过程中的变化。给药后（50 μ g/ml）， VASP磷酸化的降低与PKA活性的降低平行。为了确定苦参碱对HeLa细胞迁移的影响，我们在二维和三维细胞迁移实验中分析了HeLa细胞的迁移行为。在二维细胞迁移实验中，与对照组相比，苦参碱显著降低了细胞的平均迁移速度。此外，在用Transwell系统进行的三维细胞迁移实验中，发现用苦参碱（50 μ g/ml）处理的HeLa细胞的迁移量低于对照细胞。这些数据表明，苦参碱的抑制作用可能是通过抑制HeLa细胞粘附和迁移过程中PKA的活性而降低VASP的磷酸化而产生的。(c) 2007 Elsevier b.v.版权所有
This study sought to explore the role of matrine in the metastasis of cancer cells and to gain insight into the possible mechanism of matrine's ability to inhibit cancer metastasis. Accordingly, changes in vasodilator-stimulated phosphoprotein (VASP) phosphorylation and in cAMP-dependent protein kinase (PKA) activity during cell detachment and reattachment were first investigated. After administration of matrine (50 mu g/ml), the decrease in VASP phosphorylation paralleled the decrease in PKA activity. Matrine was found to significantly inhibit HeLa cell adhesion to collagen I. To determine the effect of matrine on the migration of HeLa cells, we analysed the migratory behaviour of HeLa cells in a two and three-dimensional cell migration assay. In a two-dimensional cell migration assay, the average cell migration velocity was significantly reduced by matrine compared with the control. Moreover, in a three-dimensional cell migration assay performed with the Transwell system, HeLa cells treated with matrine (50 mu g/ml) were found to migrate less than the control cells. These data suggest that the inhibitory effect of matrine may be produced by decreased phosphorylation of VASP due to inhibition of the activity of PKA during HeLa cell adhesion and migration. (c) 2007 Elsevier B.V All rights reserved.