Wearable multi-channel microelectrode membranes for elucidating electrophysiological phenotypes of injured myocardium
Wearable multi-channel microelectrode membranes for elucidating electrophysiological phenotypes of injured myocardium
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DOI：
10.1039/c4ib00052h
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发表时间：
2014-08-01
影响因子：
2.5
通讯作者：
Hsiai, Tzung K.
中科院分区：
文献类型：
--
作者：
Cao, Hung;Yu, Fei;Hsiai, Tzung K.
了解小型脊椎动物模型的再生能力为损伤心肌的可塑性提供了新的见解。在这里，我们展示了灵活的微电极阵列（MEA）在阐明斑马鱼和新生小鼠心脏再生模型的电生理表型的应用。4电极MEA膜被设计用于检测水生环境中的电信号。它们被微加工以粘附在斑马鱼和新生小鼠的非平面体表。对采集的信号进行处理，以显示具有高信噪比的心电图（ECG），并通过使用传统的微针电极进行验证。4通道MEA提供了信号稳定性和空间分辨率，揭示了特定部位的电损伤电流，如响应于心室冷冻损伤的ST段压低。因此，我们的基于聚合物的可穿戴MEA膜提供了对心脏损伤和再生的小椎骨模型的长期传导表型的电生理学见解，其对监测心脏病患者具有转化意义。
Understanding the regenerative capacity of small vertebrate models has provided new insights into the plasticity of injured myocardium. Here, we demonstrate the application of flexible microelectrode arrays (MEAs) in elucidating electrophysiological phenotypes of zebrafish and neonatal mouse models of heart regeneration. The 4-electrode MEA membranes were designed to detect electrical signals in the aquatic environment. They were micro-fabricated to adhere to the non-planar body surface of zebrafish and neonatal mice. The acquired signals were processed to display an electrocardiogram (ECG) with high signal-to-noise-ratios, and were validated via the use of conventional micro-needle electrodes. The 4-channel MEA provided signal stability and spatial resolution, revealing the site-specific electrical injury currents such as ST-depression in response to ventricular cryo-injury. Thus, our polymer-based and wearable MEA membranes provided electrophysiological insights into long-term conduction phenotypes for small vertebral models of heart injury and regeneration with a translational implication for monitoring cardiac patients.