Phylogenetic relationships among Oryza species revealed by AFLP markers
Phylogenetic relationships among Oryza species revealed by AFLP markers
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DOI：
10.1007/s001220051198
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发表时间：
1999-06-01
影响因子：
5.4
通讯作者：
Khush, GS
中科院分区：
文献类型：
--
作者：
Aggarwal, RK;Brar, DS;Khush, GS
栽培稻所属的稻属有22个野生种。使用扩增片段长度多态性 (AFLP) 对 23 个稻种、5 个相关属和 3 个外类群的 77 个种质进行了指纹识别。使用5个AFLP引物组合共获得1191个多态性标记。使用不同的聚类算法分析 AFLP 数据以研究物种关系，并测试所得表型图的稳定性和鲁棒性。研究结果表明，它们与稻属有共同的祖先。此外，结果表明：（1）稻的进化遵循多系路径，其中多个谱系在进化早期从共同祖先/相关类群库分离后经历了独立的分化； (2) 新分配的基因组，O. meyeriana 的 GG 和 O. Ridleyi 复合体的 HHJJ 是该属中差异最大的基因组之一，(3) CCDD 四倍体在 Officinalis 复合体中具有相对古老的起源； (4) O. malampuzhaensis、O. indandamanica、O. alta 和 O. grandiglumis 的分化足以值得获得物种地位； (5) O. officinalis 和 O. eichingeri (CC) 是 O. minuta/O 的推定祖先。分别为 malampuzhaensis 和四倍体 O. punctata，(6) O. brachyantha 是该属中分化程度最高的物种。 AFLP 是可靠的分子技术，提供了确定稻遗传关系的信息最丰富的方法之一，这对于其他相关物种/生物体也可能如此。
The genus Oryza to which cultivated rice belongs has 22 wild species. Seventy-seven accessions of 23 Oryza species, five related genera, and three outgroup taxa were fingerprinted using amplified fragment length polymorphism (AFLP). A total of 1191 polymorphic markers were obtained using five AFLP primer combinations. AFLP data were analyzed to study species relationships using different clustering algorithms, and the resulting phenograms were tested for stability and robustness. The findings suggest a common ancestry to the genus Oryza. Moreover, the results demonstrate that: (1)evolution in Oryza has followed a polyphyletic path wherein multiple lineages underwent independent divergence after separation early in the evolution from a common ancestor/pool of related taxa; (2) newly assigned genomes, GG for O. meyeriana and HHJJ for O. ridleyi complexes, are among the most diverged in the genus, (3) CCDD tetraploids have a relatively ancient origin among the Officinalis complex; (4) O. malampuzhaensis, O. indandamanica, O. alta, and O. grandiglumis are diverged enough to deserve species status; (5) O. officinalis and O. eichingeri (CC) are putative progenitors of O. minuta/O. malampuzhaensis and tetraploid O. punctata, respectively, (6) O. brachyantha is most diverged species in the genus. AFLP is reliable molecular technique and provides one of the most informative approaches to ascertain genetic relationships in Oryza, which may also be true for other related species/organisms.