Effects of Light Intensity and Exposure Period on the Growth and Stress Responses of Two Cyanobacteria Species: Pseudanabaena galeata and Microcystis aeruginosa
Effects of Light Intensity and Exposure Period on the Growth and Stress Responses of Two Cyanobacteria Species: Pseudanabaena galeata and Microcystis aeruginosa
复制标题
DOI：
10.3390/w12020407
复制
发表时间：
2020-02
期刊：
影响因子：
3.4
通讯作者：
Guligena Muhetaer;T. Asaeda;Senavirathna M. D. H. Jayasanka;Mahendra B. Baniya;H. D. L. Abeynayaka;M. H. Ras
中科院分区：
文献类型：
--
作者：
Guligena Muhetaer;T. Asaeda;Senavirathna M. D. H. Jayasanka;Mahendra B. Baniya;H. D. L. Abeynayaka;M. H. Ras
光照是影响蓝藻生长的重要因素，光照的变化会影响蓝藻的生长和生理。然而，光诱导的氧化应激和蓝藻在不同光照水平下的物种特异性行为存在信息缺口。本研究进行了评估的比较影响不同的光照强度的生长和两种蓝藻，Pseudanabaena galeata（菌株NIES 512）和铜绿微囊藻（菌株NIES 111），后2天和8天的压力响应。蓝藻培养物在以下不同的光照强度下生长：0、10、30、50、100、300和600 μmol m−2 s−1。分别测定了各藻种的光密度（OD 730）、叶绿素a（Chl-a）含量、蛋白质含量、H2 O2含量以及过氧化氢酶（CAT）和过氧化物酶（POD）的抗氧化酶活性。当光照强度低于30 μmol m−2 s−1和高于50 μmol m−2 s−1时，盔状原球茎会受到负面影响。30 ~ 50 μmol m-2s-1的光照有利于盔状原球茎的生长，而M.铜绿假单胞菌对极端光照条件具有更高的耐受性。M.铜绿假单胞菌为10至100 μmol m−2 s−1。
Light is an important factor that affects cyanobacterial growth and changes in light can influence their growth and physiology. However, an information gap exists regarding light-induced oxidative stress and the species-specific behavior of cyanobacteria under various light levels. This study was conducted to evaluate the comparative effects of different light intensities on the growth and stress responses of two cyanobacteria species, Pseudanabaena galeata (strain NIES 512) and Microcystis aeruginosa (strain NIES 111), after periods of two and eight days. The cyanobacterial cultures were grown under the following different light intensities: 0, 10, 30, 50, 100, 300, and 600 μmol m−2 s−1. The optical density (OD730), chlorophyll a (Chl-a) content, protein content, H2O2 content, and the antioxidative enzyme activities of catalase (CAT) and peroxidase (POD) were measured separately in each cyanobacteria species. P. galeata was negatively affected by light intensities lower than 30 μmol m−2 s−1 and higher than 50 μmol m−2 s−1. A range of 30 to 50 μmol m−2 s−1 light was favorable for the growth of P. galeata, whereas M. aeruginosa had a higher tolerance for extreme light conditions. The favorable range for M. aeruginosa was 10 to 100 μmol m−2 s−1.