MALE-SPECIFIC, SEX PHEROMONE-SELECTIVE PROJECTION NEURONS IN THE ANTENNAL LOBES OF THE MOTH MANDUCASEXTA
MALE-SPECIFIC, SEX PHEROMONE-SELECTIVE PROJECTION NEURONS IN THE ANTENNAL LOBES OF THE MOTH MANDUCASEXTA
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DOI：
10.1007/bf00611929
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发表时间：
1987-01-01
影响因子：
2.1
通讯作者：
HILDEBRAND, JG
中科院分区：
文献类型：
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作者：
CHRISTENSEN, TA;HILDEBRAND, JG
嗅觉投射神经元的一个子集在男性天蛾大脑中的描述，和他们的作用，性信息素的信息处理进行检查。这些神经元在巨球复合体（MGC）中有广泛的分支，MGC是触角叶（AL）中神经元的一个独特的性二态区域，两种已知的触角信息素受体的轴突投射到MGC。每个投射神经元都将一个轴突从AL发送到前脑。41投射神经元的特点是根据他们的反应，电刺激触角神经以及嗅觉刺激触角受体。所有神经元对雌性性信息素都表现出很强的选择性。其他行为相关的气味，如植物挥发物，对这些神经元的活动没有明显的影响。发现了两大生理类别：（a）通过用信息素刺激同侧天线而兴奋的细胞（41个中的29个），以及（B）从信息素途径接收混合输入（抑制和兴奋）的细胞（41个中的12个）。在第一类细胞中，29个细胞中有13个对主要天然信息素（bombykal）或第二种未识别信息素（“C-15”）的模拟物刺激天线同样兴奋，并且同样受到天然信息素混合物的刺激。剩下的16个29个细胞选择性地响应，在某些情况下，以剂量依赖性的方式，刺激的天线与bombykal或C-15，但不是两者兼而有之。其中一些神经元的树突arborizations仅限于一部分的MGC神经元，而大多数arborizizations整个MGC。在第二类中的细胞中，12个中有9个被Bombykal兴奋，被C-15抑制，并且对天然信息素混合物表现出混合反应。对于12个细胞中的另外3个，对于两种化学鉴定的气味的响应极性是相反的。另外两个神经元，这是没有测试嗅觉刺激，张力抑制响应于电刺激同侧触角神经。这些观察结果表明，一些男性特定的投射神经元可能会发出一般信息素触发的唤醒信号，而较少的数量可以积极整合来自两个已知的受体类别（Bal和C-15选择性）的输入，并可能在处理有关性信息素的嗅觉子系统的这一水平上作为“混合物检测器”。
A subset of olfactory projection neurons in the brain of male Manduca sexta is described, and their role in sex pheromone information processing is examined. These neurons have extensive arborizations in the macroglomerular complex (MGC), a distinctive and sexually dimorphic area of neuropil in the antennal lobe (AL), to which the axons of two known classes of antennal pheromone receptors project. Each projection neuron sends an axon from the AL into the protocerebrum. Forty-one projection neurons were characterized according to their responses to electrical stimulation of the antennal nerve as well as olfactory stimulation of antennal receptors. All neurons exhibited strong selectivity for female sex pheromones. Other behaviorally relevant odors, such as plant volatiles, had no obvious effect on the activity of these neurons. Two broad physiological categories were found: (a) cells that were excited by stimulation of the ipsilateral antenna with pheromones (29 out of 41), and (b) cells that received a mixed input (inhibition and excitation) from pheromone pathways (12 out of 41). Of the cells in the firt category, 13 out of 29 were equally excited in response to stimulation of the antenna with either the principal natural pheromone (bombykal) or a mimic of a second unidentified pheromone (''C-15'') and were similarly excited by the natural pheromone blend. The remaining 16 out of 29 cells responded selectively, and in some cases, in a dose-dependent manner, to stimulation of the antenna with bombykal or C-15, but not both. Some of these neurons had dendritic arborizations restricted to only a portion of the MGC neuropil, whereas most had arboriziations throughout the MGC. Of the cells in the second category, 9 out of 12 were excited by bombykal, inhibited by C-15, and showed a mixed response to the natural pheromone blend. For the other 3 out of 12 cells, the response polarity was reversed for the two chemically-identified odors. Two additional neurons, which were not tested with olfactory stimuli, were tonically inhibited in response to electrical stimulation of the ipsilateral antennal nerve. These observations suggest that some of the male-specific projection neurons may signal general pheromone-triggered arousal, whereas a smaller number can actively integrate inputs from the two known receptor classes (Bal- and C-15-selective) and may operate as ''mixture detectors'' at this level of the olfactory subsystem that processes information about sex pheromones.