BEHAVIORAL INVESTIGATION OF THE COEXISTENCE OF SUBSTANCE-P, CORTICOTROPIN RELEASING-FACTOR, AND ACETYLCHOLINESTERASE IN LATERAL DORSAL TEGMENTAL NEURONS PROJECTING TO THE MEDIAL FRONTAL-CORTEX OF THE RAT
BEHAVIORAL INVESTIGATION OF THE COEXISTENCE OF SUBSTANCE-P, CORTICOTROPIN RELEASING-FACTOR, AND ACETYLCHOLINESTERASE IN LATERAL DORSAL TEGMENTAL NEURONS PROJECTING TO THE MEDIAL FRONTAL-CORTEX OF THE RAT
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DOI：
10.1016/0196-9781(85)90320-1
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发表时间：
1985-01-01
期刊：
影响因子：
3
通讯作者：
JACOBOWITZ, DM
中科院分区：
文献类型：
--
作者：
CRAWLEY, JN;OLSCHOWKA, JA;JACOBOWITZ, DM
用逆行追踪和免疫细胞化学染色方法，观察了P物质（SP）、促肾上腺皮质激素释放因子（CRF）和乙酰胆碱酯酶（AChE）在大鼠背外侧被盖核（ntdl）向内侧额叶皮质（MFC）、隔和丘脑投射的共定位。组织化学结果表明，SP和CRF共存的一个亚群的NTDL胆碱能神经元，项目的一些前脑区域，包括MFC。行为学研究的影响，SP，CRF，和胆碱能激动剂，卡巴胆碱，采用微量注射到大鼠的MFC，SP和CRF没有引起任何行为的影响时，单独给药。卡巴胆碱（1-5 μ g/侧）产生定型的运动行为，包括在直立姿势时快速前爪踩踏，类似于“拳击”。SP（1 μ g/侧）增加卡巴胆碱诱导的“拳击“。“CRF（1-10 ng/侧）减少卡巴胆碱诱导的“拳击。“SP、CRF和乙酰胆碱酯酶共存的一个可能的功能意义，在投射到大鼠内侧额叶皮质的神经元中，似乎是SP对胆碱能反应的调节增强，和CRF对胆碱能反应的调节抑制。
Colocalization of substance P (SP), corticotropin releasing factor (CRF), and acetylcholinesterase (AChE) was detected by retrograde tracing and immunocytochemical staining in the nucleus tegmentalis dorsalis lateralis (ntdl) projecting to the medial frontal cortex (MFC), septum, and thalamus of the rat. The histochemical results suggest that SP and CRF coexist within a subpopulation of ntdl cholinergic neurons that project to a number of forebrain regions including the MFC. Behavioral studies of the effects of SP, CRF, and the cholinergic agonist, carbachol, employed microinjections into the MFC of rats, SP and CRF did not elicit any behavioral effects when administered alone. Carbachol (1-5 .mu.g/side) produced a stereotyped motor behavior, consisting of rapid forepaw treading while in an upright posture, resembling "boxing." SP (1 .mu.g/side) increased carbachol-induced "boxing." CRF (1-10 ng/side) decreased carbachol-induced "boxing." One possible functional significance of the coexistence of SP, CRF, and acetylcholinesterase, in neurons projecting to the medial frontal cortex in rats, appears to be a modulatory potentiation of cholinergic response by SP, and a modulatory inhibition of the cholinergic response by CRF.