MECHANICAL TRANSMISSION, PURIFICATION, AND SOME PROPERTIES OF WHITEFLY-BORNE MUNG BEAN YELLOW MOSAIC-VIRUS IN THAILAND
MECHANICAL TRANSMISSION, PURIFICATION, AND SOME PROPERTIES OF WHITEFLY-BORNE MUNG BEAN YELLOW MOSAIC-VIRUS IN THAILAND
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DOI：
10.1094/pd-67-801
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发表时间：
1983-01-01
期刊：
影响因子：
4.5
通讯作者：
DEEMA, N
中科院分区：
文献类型：
--
作者：
HONDA, Y;IWAKI, M;DEEMA, N
在泰国，粉虱传绿豆黄花叶病毒（MYMV）是通过机械接种传播的。在尝试传输中使用的几种缓冲液中，0.1 M磷酸钾或磷酸钠（pH 7.8）的传输率最高。症状表达的最佳温育温度范围为25 - 30 ℃。生长室中的温度为30 ℃或30 ℃。白天为20 ℃，晚上在温室里。MYMV的寄主范围仅限于豆科7种植物。测定植物汁液中病毒的稳定性，得到以下结果：热灭活点为40 ℃。50.degree.稀释终点为10-2-10-3，20 ℃下体外寿命为1-2天。C.纯化的病毒制剂具有核蛋白典型的紫外光吸收光谱，A260/A280值为λ。1.3-1.4.纯化的制剂和浸叶样品含有约的成对颗粒。18倍30纳米。免疫活性与纯化的病毒颗粒的存在有关。
The whitefly-borne mung bean yellow mosaic virus (MYMV) in Thailand was transmitted by mechanical inoculation. Among the several buffers used in attempted transmissions, 0.1 M potassium or sodium phosphate, pH 7.8, gave the highest transmission rates. The optimal incubation temperatures for symptom expression ranged from 25-30.degree. C in the growth chamber or 30.degree. C in the daytime and 20.degree. C at night in the greenhouse. Host range of MYMV was limited to 7 plant species in the family Leguminosae. Determinations of the stability of the virus in plant sap gave the following results: thermal inactivation point of 40.degree.-50.degree. C for 10 min, dilution end point of 10-2-10-3 and longevity in vitro of 1-2 days at 20.degree. C. Purified virus preparations had an UV light absorption spectrum typical of that of nucleoprotein with a A260/A280 value of .apprx. 1.3-1.4. Purified preparations and leaf-dip samples contained geminate particles of .apprx. 18 .times. 30 nm. Infectivity was associated with the presence of purified virus particles.