Temporal patterns of gene expression in developing maize endosperm identified through transcriptome sequencing
Temporal patterns of gene expression in developing maize endosperm identified through transcriptome sequencing
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DOI：
10.1073/pnas.1406383111
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发表时间：
2014-05-27
影响因子：
11.1
通讯作者：
Yadegari, Ramin
中科院分区：
文献类型：
--
作者：
Li, Guosheng;Wang, Dongfang;Yadegari, Ramin
胚乳是被子植物发生第二次受精后形成的子代结构。胚乳作为一种可吸收的贮藏器官，在胚胎发育和幼苗萌发中起着至关重要的作用。谷物胚乳中碳水化合物和蛋白质储存产物的积累为人类提供了大部分的食物、饲料和可再生资源。关于控制胚乳增殖和分化的调控基因网络，人们知之甚少。作为了解这些网络的第一步，我们在授粉后的第一个12d期间连续分析了玉米籽粒和胚乳中的所有mRNAs。对这些基因集的分析确定了基因表达的时间程序，包括数百个转录因子基因。我们发现，在发育中的胚乳的不同隔室中，顺序表达的基因集与不同的细胞和代谢程序密切相关。这些结果构成了胚乳基因表达的时空模式的初步图谱，以支持未来的努力，以了解控制种子产量和质量的潜在机制。
Endosperm is a filial structure resulting from a second fertilization event in angiosperms. As an absorptive storage organ, endosperm plays an essential role in support of embryo development and seedling germination. The accumulation of carbohydrate and protein storage products in cereal endosperm provides humanity with a major portion of its food, feed, and renewable resources. Little is known regarding the regulatory gene networks controlling endosperm proliferation and differentiation. As a first step toward understanding these networks, we profiled all mRNAs in the maize kernel and endosperm at eight successive stages during the first 12 d after pollination. Analysis of these gene sets identified temporal programs of gene expression, including hundreds of transcription-factor genes. We found a close correlation of the sequentially expressed gene sets with distinct cellular and metabolic programs in distinct compartments of the developing endosperm. The results constitute a preliminary atlas of spatiotemporal patterns of endosperm gene expression in support of future efforts for understanding the underlying mechanisms that control seed yield and quality.