EARLY ADRENALECTOMY STIMULATES SUBSEQUENT GROWTH AND DEVELOPMENT OF THE RAT-BRAIN
EARLY ADRENALECTOMY STIMULATES SUBSEQUENT GROWTH AND DEVELOPMENT OF THE RAT-BRAIN
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DOI：
10.1016/0014-4886(83)90415-6
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
MEYER, JS
中科院分区：
文献类型：
--
作者：
MEYER, JS
分别于生后第11天摘除肾上腺(ADX)或假手术(Sham)，观察大鼠脑发育(大脑和小脑)的组织重量和生化组成。在大约65天大的时候测量，早期ADX受试者的大脑(就湿重和干重而言)明显比Sham组重，尽管总体体重较轻。脑蛋白质和DNA含量升高，髓鞘标志酶2‘’，3‘-环核苷酸3’-磷酸二酯酶(CNP)活性也升高。糖皮质激素调节的甘油-3-磷酸脱氢酶(GPDH)活性降低。虽然大脑和小脑都表现出早期肾上腺切除的促生长作用，但DNA和CNP的变化在大脑中最为明显。亚细胞分离实验证实了摘除肾上腺对髓鞘生成的影响，表明ADX组大鼠脑内髓鞘的含量高于假手术组。这些结果在肾上腺分泌物对正常脑发育的影响以及GPDH在髓鞘脂质生物合成中的作用方面具有重要意义。
Rats were adrenalectomized (ADX) or sham-operated (SHAM) on the 11th day of life and subsequent brain development (cerebrum and cerebellum) studied in terms of tissue weight and biochemical composition. Measured at about 65 days of age, early ADX subjects had significantly heavier brains (in terms of both wet and dry weights) than SHAM, despite being lighter in overall body weight. Brain protein and DNA contents were elevated, as was the activity of 2'',3''-cyclic nucleotide 3''-phosphodiesterase(CNP), a myelin marker enzyme. Glycerol-3-phosphate dehydrogenase (GPDH), a glucocorticoid-regulated enzyme, was reduced in activity. Although both the cerebrum and cerebellum showed the growth-enhancing effects of early adrenalectomy, the DNA and CNP changes were most pronounced in the cerebrum. The effect of adrenal removal on myelinogenesis was confirmed by subcellular fractionation experiments, demonstrating that more myelin could be recovered from the brains of ADX than from SHAM animals. These results are significant in terms of the influence of adrenal secretions on normal brain development and the role of GPDH in myelin lipid biosynthesis.