Comparative study of the chemotactic responses of periodontal ligament cells and gingival fibroblasts to polypeptide growth factors
Comparative study of the chemotactic responses of periodontal ligament cells and gingival fibroblasts to polypeptide growth factors
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DOI：
10.1177/00220345960750041401
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发表时间：
1996-04-01
影响因子：
7.6
通讯作者：
Terranova, VP
中科院分区：
文献类型：
--
作者：
Nishimura, F;Terranova, VP
牙周膜细胞选择性募集到先前暴露的牙根表面被认为可以增强牙周再生。据推测，来自牙龈成纤维细胞的竞争可能会降低牙周再生的潜力。我们比较了PDL细胞和牙龈成纤维细胞对各种生物制剂的迁移反应。平行实验旨在检查牙周膜细胞的定向迁移反应，使用血小板衍生生长因子（PDGF）、胰岛素样生长因子-I、-II、胰岛素样生长因子-I、胰岛素样生长因子-II、胰岛素样生长因子-III、胰岛素样生长因子-IV、胰岛素样生长因子IV（IGF-I，-II），表皮生长因子（EGF），转化生长因子-β当用PDGF、IGF-I、IGF-II、EGF和TGF-β攻击时，PDL细胞和GF都表现出剂量依赖性的迁移反应。然而，当这些细胞与PDL-CTX的挑战，只有PDL细胞迁移在一个特定的剂量依赖性的方式，而GF是折射PDL-CTX刺激。此外，牙龈成纤维细胞培养物的浓缩条件培养基不刺激PDL细胞迁移反应。在其他实验中，针对PDGF、FGF、TGF-β、IGF-I、IGF-II、NGF和EGF的抗体不抑制PDL-CTX在PDL细胞中引起的应答。在这份手稿中提出的数据表明，牙周膜细胞和牙龈成纤维细胞响应多种生长因子。发现PDL-CTX对于定向迁移是PDL细胞特异性的。因此，我们得出结论，任何生物治疗牙周再生制度应包括PDL细胞特异性药物。
Selective recruitment of periodontal ligament cells to a previously exposed root surface is believed to enhance periodontal regeneration. It has been hypothesized that competition from gingival fibroblasts may reduce the potential of periodontal regeneration. We compared the migratory responses of PDL cells and gingival fibroblasts to a variety of biologicals. Parallel experiments designed to examine the directed migration responses of both periodontal ligament cells (PDL cells) and gingival fibroblasts (GF) isolated from the same donors were conducted using Platelet Derived Growth Factor (PDGF), Insulin Like Growth Factor-I, -II (IGF-I, -II), Epidermal Growth Factor (EGF), Transforming Growth Factor-beta (TGF-beta), and the chemotactic factor derived from the conditioned culture media of PDL cells (termed PDL-CTX) as attractants.Both PDL cells and GF exhibited dose-dependent migratory responses when challenged with PDGF, IGF-I, IGF-II, EGF, and TGF-beta. However, when these cells were challenged with PDL-CTX, only PDL cells migrated in a specific dose-dependent manner, while GF were refractive to PDL-CTX stimulation. Additionally, concentrated conditioned culture media from cultures of gingival fibroblasts did not stimulate PDL cell migratory responses. In other experiments, antibody directed against PDGF, FGF, TGF-beta, IGF-I, IGF-II, NGF, and EGF did not inhibit the PDL-CTX-elicited response in PDL cells.Previous studies have suggested that success of periodontal therapy depends on the specific attachment, migration, and proliferation of selected periodontal ligament cells. The data presented in this manuscript suggest that both PDL cells and gingival fibroblasts respond to a multitude of growth factors. PDL-CTX was found to be PDL-cell-specific for directed migration. Thus, we conclude that any biological therapeutic regime for periodontal regeneration should include PDL-cell-specific agents.