Using source-associated mobile genetic elements to identify zoonotic extraintestinal E. coli infections.
Using source-associated mobile genetic elements to identify zoonotic extraintestinal E. coli infections.
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DOI：
10.1016/j.onehlt.2023.100518
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发表时间：
2023-06
期刊：
影响因子：
5
通讯作者：
Price, Lance B
中科院分区：
文献类型：
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作者：
Liu, Cindy M;Aziz, Maliha;Park, Daniel E;Wu, Zhenke;Stegger, Marc;Li, Mengbing;Wang, Yashan;Schmidlin, Kara;Johnson, Timothy J;Koch, Benjamin J;Hungate, Bruce A;Nordstrom, Lora;Gauld, Lori;Weaver, Brett;Rolland, Diana;Statham, Sally;Hall, Brantley;Sariya, Sanjeev;Davis, Gregg S;Keim, Paul S;Johnson, James R;Price, Lance B
“同一健康”视角可能为大肠杆菌传播提供新的且可付诸行动的信息。大肠杆菌在包括人类和食用动物在内的多种脊椎动物体内定殖，是人类膀胱、肾脏和血液感染的主要病因。大量证据支持致病性大肠杆菌菌株从食用动物到人类的食源传播。然而，食源性人畜共患大肠杆菌（FZEC）对人类肠外疾病负担的相对贡献以及此类菌株的显著特征仍未明确。通过对大量同时期、地理匹配的临床和肉类来源的大肠杆菌分离株（n = 3111）进行比较基因组分析，我们确定了17种与来源相关的可移动遗传元件——主要是质粒和噬菌体——并将它们整合到一种新的贝叶斯潜在类别模型中，以预测临床大肠杆菌分离株的来源。我们估计，在我们的研究人群中，约8%的人类肠外大肠杆菌感染（主要是尿路感染）是由FZEC引起的。FZEC菌株与非FZEC菌株引发症状性疾病的可能性相同。两个FZEC谱系，ST131 - H22和ST58，似乎具有特别高的毒力潜能。我们的研究结果表明，FZEC菌株总体上引起的尿路感染比任何一种非大肠杆菌的尿路致病菌（例如肺炎克雷伯菌）都要多。我们的新方法可应用于其他环境，以识别风险最高的FZEC菌株，确定其来源，并为新的“同一健康”策略提供信息，以减轻肠外大肠杆菌感染带来的沉重公共卫生负担。
肉类可能是人类接触食用动物肠外致病性大肠杆菌菌株的重要媒介。
我们提出了一种预测临床大肠杆菌分离株来源的新方法。
在我们的人群中，约8%的临床大肠杆菌分离株似乎是食源性人畜共患病菌株。
食源性人畜共患大肠杆菌菌株与无症状菌尿、尿路感染和败血症有关。
我们估计，食源性人畜共患大肠杆菌菌株每年在美国导致超过48万例尿路感染。
A one-health perspective may provide new and actionable information about Escherichia coli transmission. E. coli colonizes a broad range of vertebrates, including humans and food-production animals, and is a leading cause of bladder, kidney, and bloodstream infections in humans. Substantial evidence supports foodborne transmission of pathogenic E. coli strains from food animals to humans. However, the relative contribution of foodborne zoonotic E. coli (FZEC) to the human extraintestinal disease burden and the distinguishing characteristics of such strains remain undefined. Using a comparative genomic analysis of a large collection of contemporaneous, geographically-matched clinical and meat-source E. coli isolates (n = 3111), we identified 17 source-associated mobile genetic elements – predominantly plasmids and bacteriophages – and integrated them into a novel Bayesian latent class model to predict the origins of clinical E. coli isolates. We estimated that approximately 8 % of human extraintestinal E. coli infections (mostly urinary tract infections) in our study population were caused by FZEC. FZEC strains were equally likely to cause symptomatic disease as non-FZEC strains. Two FZEC lineages, ST131-H22 and ST58, appeared to have particularly high virulence potential. Our findings imply that FZEC strains collectively cause more urinary tract infections than does any single non-E. coli uropathogenic species (e.g., Klebsiella pneumoniae). Our novel approach can be applied in other settings to identify the highest-risk FZEC strains, determine their sources, and inform new one-health strategies to decrease the heavy public health burden imposed by extraintestinal E. coli infections. Meat may be an important vehicle for human exposure to extraintestinal pathogenic E. coli strains from food animals. We present a novel approach for predicting the origins of clinical E. coli isolates. Approximately 8% of the clinical E. coli isolates in our population appeared to be foodborne zoonotic strains. Foodborne zoonotic E. coli strains were associated with asymptomatic bacteriuria, urinary tract infections, and sepsis. We estimate that foodborne zoonotic E. coli strains cause >480,000 urinary tract infections in the U.S. annually.