High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis
High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis
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DOI：
10.1023/a:1023997627578
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发表时间：
2003-08-01
影响因子：
3
通讯作者：
Liu, JR
中科院分区：
文献类型：
--
作者：
Kim, SW;In, DS;Liu, JR
以春水草（Hylomecon vernalis Max.）为材料，研究了其叶柄、叶片外植体培养和叶柄胚性细胞悬浮培养的体细胞胚胎发生和植株再生的培养条件。叶柄外植体形成胚性愈伤组织的频率为53%时，培养在B5培养基上补充了13.6 μ M的2，4-二氯苯氧乙酸（2，4-D）单独。当在4.52 μ M的2，4-D和2.22 μ M的6-苄基腺嘌呤的组合培养时，叶外植体以21%的频率形成胚性愈伤组织。用含有4.52 μ M 2，4-D的液体B5培养基，用叶柄衍生的胚性愈伤组织建立细胞悬浮培养。在接种到B5基础培养基上时，细胞悬浮培养物产生许多体细胞胚，然后发育成小植株。将再生的小植株移植到盆栽土壤中并在温室中生长至成熟。
Culture conditions are described for high frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis Max. Petiole explants formed embryogenic calluses at a frequency of 53% when cultured on B5 medium supplemented with 13.6 muM 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Leaf explants formed embryogenic calluses at a frequency of 21% when cultured at a combination of 4.52 muM 2,4-D and 2.22 muM 6-benzyladenine. Cell suspension cultures were established with petiole-derived embryogenic calluses using liquid B5 medium with 4.52 muM 2,4-D. Upon plating onto B5 basal medium, cell suspension cultures produced numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.