A high-fat, ketogenic diet induces a unique metabolic state in mice
A high-fat, ketogenic diet induces a unique metabolic state in mice
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DOI：
10.1152/ajpendo.00717.2006
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发表时间：
2007-06-01
影响因子：
5.1
通讯作者：
Maratos-Flier, Eleftheria
中科院分区：
文献类型：
--
作者：
Kennedy, Adam R.;Pissios, Pavlos;Maratos-Flier, Eleftheria
基于低碳水化合物、高脂肪饮食的理论优势，生酮饮食已被用作减肥方法。为了评估此类饮食对体重的生理和代谢影响，我们研究了食用极低碳水化合物生酮饮食 (KD) 的小鼠。这种饮食对能量平衡和基因表达具有深远的影响。 C57BL/6小鼠动物被喂食四种饮食之一：KD；常用的致肥高脂肪、高蔗糖饮食（HF）； 66% 热量限制 (CR)；并控制食物（C）。服用 KD 的小鼠与服用 C 和 HF 的小鼠摄入的热量相同，但体重下降并稳定在初始体重的 85%，与 CR 类似。这与喂食 KD 的动物与喂食 C 和 CR 的动物相比能量消耗增加是一致的。肝脏的微阵列分析显示 KD 中独特的基因表达模式，脂肪酸氧化途径中的基因表达增加，脂质合成途径中的基因表达减少。因 HF 导致肥胖并转变为 KD 的动物失去了所有多余的体重，改善了葡萄糖耐量，并增加了能量消耗。对关键基因的分析显示，与直接服用生酮饮食的瘦动物中观察到的变化类似。此外，AMP 激酶活性增加，ACC 活性相应降低。这些数据表明，生酮饮食会诱导一种与减肥相一致的独特代谢状态。
Ketogenic diets have been used as an approach to weight loss on the basis of the theoretical advantage of a low- carbohydrate, high- fat diet. To evaluate the physiological and metabolic effects of such diets on weight we studied mice consuming a very- low- carbohydrate, ketogenic diet ( KD). This diet had profound effects on energy balance and gene expression. C57BL/ 6 mice animals were fed one of four diets: KD; a commonly used obesogenic high- fat, high- sucrose diet ( HF); 66% caloric restriction ( CR); and control chow ( C). Mice on KD ate the same calories as mice on C and HF, but weight dropped and stabilized at 85% initial weight, similar to CR. This was consistent with increased energy expenditure seen in animals fed KD vs. those on C and CR. Microarray analysis of liver showed a unique pattern of gene expression in KD, with increased expression of genes in fatty acid oxidation pathways and reduction in lipid synthesis pathways. Animals made obese on HF and transitioned to KD lost all excess body weight, improved glucose tolerance, and increased energy expenditure. Analysis of key genes showed similar changes as those seen in lean animals placed directly on KD. Additionally, AMP kinase activity was increased, with a corresponding decrease in ACC activity. These data indicate that KD induces a unique metabolic state congruous with weight loss.