Expression analysis of osteopontin mRNA splice variants in prostate cancer and benign prostatic hyperplasia
Expression analysis of osteopontin mRNA splice variants in prostate cancer and benign prostatic hyperplasia
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DOI：
10.1016/j.yexmp.2011.09.014
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Gimba, E. R.
中科院分区：
文献类型：
--
作者：
Tilli, T. M.;Thuler, L. C.;Gimba, E. R.
骨桥蛋白剪接异构体(OPN-SI)呈现不同的表达模式和特定的肿瘤作用。我们的目的是研究OPN-SI在前列腺癌(PCa)和良性前列腺增生症(BPH)组织中的表达特征，并评估它们作为前列腺癌诊断和预后意义的生物标志物的可能性。前列腺癌患者40例，良性前列腺增生症(BPH)患者30例。采用实时定量聚合酶链式反应(qRT-PCR)检测OPN-SI基因的表达。用抗OPNc多克隆抗体进行免疫组织化学分析。生物统计学分析评估了OPN-SI和总前列腺特异性抗原(PSA)血清水平与临床和病理数据的关系。PCa组织样本的OPNa水平显著高于对照组。OPNb和OPNc文字记录(p
Osteopontin splicing isoforms (OPN-SI) present differential expression patterns and specific tumor roles. Our aims were to characterize OPN-SI expression in prostate cancer (PCa) and benign prostate hyperplasia (BPH) tissues, besides evaluating their potential as biomarkers for PCa diagnosis and prognostic implications. Prostatic tissue specimens were obtained from 40 PCa and 30 benign prostate hyperplasia (BPH) patients. Quantitative real time PCR (qRT-PCR) was used to measure OPN-SI mRNA expression. Immunohistochemical analysis was performed using an anti-OPNc polyclonal antibody. Biostatistical analyses evaluated the association of OPN-SI and total Prostate Specific Antigen (PSA) serum levels with clinical and pathological data. PCa tissue samples presented significantly higher levels of OPNa. OPNb and OPNc transcripts (p