Characterization of the interaction of a novel Stagonospora nodorum host-selective toxin with a wheat susceptibility gene
Characterization of the interaction of a novel Stagonospora nodorum host-selective toxin with a wheat susceptibility gene
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DOI：
10.1104/pp.107.108761
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发表时间：
2008-02-01
期刊：
影响因子：
7.4
通讯作者：
Faris, Justin D.
中科院分区：
文献类型：
--
作者：
Friesen, Timothy L.;Zhang, Zengcui;Faris, Justin D.
最近的研究表明，Stagonospora nodorum小麦致病系统是由主机选择性毒素（HSTs; SnToxA，SnTox 1，和SnTox 2），直接或间接地与显性宿主基因（Tsn 1，Snn 1，和Snn 2）相互作用，以诱导疾病。在这里，我们描述和表征一种新的HST命名为SnTox 3，和相应的小麦敏感性/易感性基因上的染色体臂5 BS，我们指定为Snn 3。SnTox 3是一种大小在10和30 kD之间的蛋白质性坏死诱导毒素。色葡萄在小麦重组近交系群体上评估了黑瘤病分离物Sn 1501（SnToxA-、SnTox 2+和SnTox 3+）、SN 15（SnToxA+、SnTox 2+和SnTox 3+）和SN 15 KO 18（具有破坏形式的SnToxA的SN 15菌株）。一个兼容的Snn 3-SnTox 3的相互作用发挥了重要作用的发展所造成的疾病的分离物Sn 1501和SN 15 KO 18，与Snn 2是上位性的Snn 3。Snn 3与SN 15引起的疾病没有显著相关性，这可能是由于Snn 2和Tsn 1观察到的主要效应，它们在很大程度上是加性的。本文介绍了S. nodorum的概念，并建立在S.根瘤菌-小麦致病系统在很大程度上是基于多个宿主-毒素相互作用，遵循逆基因对基因的情况。
Recent work suggests that the Stagonospora nodorum-wheat pathosystem is controlled by host-selective toxins (HSTs; SnToxA, SnTox1, and SnTox2) that interact directly or indirectly with dominant host genes (Tsn1, Snn1, and Snn2) to induce disease. Here we describe and characterize a novel HST designated SnTox3, and the corresponding wheat sensitivity/susceptibility gene identified on chromosome arm 5BS, which we designated as Snn3. SnTox3 is a proteinaceous necrosis-inducing toxin between 10 and 30 kD in size. The S. nodorum isolates Sn1501 (SnToxA-, SnTox2+, and SnTox3+), SN15 (SnToxA+, SnTox2+, and SnTox3+), and SN15KO18, a strain of SN15 with a disrupted form of SnToxA, were evaluated on a population of wheat recombinant inbred lines. A compatible Snn3-SnTox3 interaction played a significant role in the development of disease caused by isolates Sn1501 and SN15KO18, with Snn2 being epistatic to Snn3. Snn3 was not significantly associated with disease caused by SN15 presumably due to the major effects observed for Snn2 and Tsn1, which were largely additive. This work introduces a fourth HST produced by S. nodorum and builds on the notion that the S. nodorum-wheat pathosystem is largely based on multiple host-toxin interactions that follow an inverse gene-for-gene scenario.