Experience with a Simple High-Performance Liquid Chromatography Method for the Analysis of Purine and Pyrimidine Nucleosides and Bases in Biological Fluids
Experience with a Simple High-Performance Liquid Chromatography Method for the Analysis of Purine and Pyrimidine Nucleosides and Bases in Biological Fluids
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使用简单的高效液相色谱方法分析生物液体中的嘌呤和嘧啶核苷和碱基的经验
DOI：
10.1177/000456328402100210
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发表时间：
1984
影响因子：
2.2
通讯作者：
K. Carpenter
中科院分区：
文献类型：
--
作者：
M. Bennett;K. Carpenter
使用等规反相高效液相色谱技术和简单的样品制备技术分析了嘌呤和嘧啶核苷和碱基。该方法已成功地用于在30分钟内分离大量生物产生的核酸产物，并通过它们的层析和光学性质来鉴定它们。一些代谢紊乱已被确定，包括次黄嘌呤-鸟嘌呤磷酸核糖转移酶和鸟氨酸氨基甲酰转移酶缺乏症。该方法已常规使用2年，进行了定量和定性分析，证明了该方法的可靠性和可靠性。
Purine and pyrimidine nucleosides and bases were analysed using an isocratic reverse-phase HPLC technique and utilising simple sample preparation. The method has been successfully used to separate a large number of biologically occurring nucleic acid products within a 30-minute period and to identify them by their chromatographic and optical properties. A number of metabolic disorders have been identified, including hypoxanthine-guanine phosphoribosyl transferase and ornithine carbamyl transferase deficiencies. The method has been in routine use for 2 years performing both quantitative and qualitative analysis and has proved to be robust and reliable.