A fast liquid chromatographic/mass spectrometric screening method for the simultaneous detection of synthetic glucocorticoids, some stimulants, anti-olestrogen drugs and synthetic anabolic steroids
A fast liquid chromatographic/mass spectrometric screening method for the simultaneous detection of synthetic glucocorticoids, some stimulants, anti-olestrogen drugs and synthetic anabolic steroids
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DOI：
10.1002/rcm.2729
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发表时间：
2006-01-01
影响因子：
2
通讯作者：
Botre, Francesco
中科院分区：
文献类型：
--
作者：
Mazzarino, Monica;Botre, Francesco
描述了一种快速液相色谱/质谱（LC/MS/MS）筛选方法，用于检测尿液中合成糖皮质激素、兴奋剂（福莫特罗、莫达非尼和mesocarb）、抗雌激素（非那雄胺、阿司美坦、阿那曲唑、来曲唑和福美坦）和合成合成代谢类固醇（司坦唑醇、孕三烯酮和四氢孕三烯酮）。所有这些药物（和/或其尿液代谢物）可通过单一液/液提取步骤，在碱性pH下，在β-葡萄糖醛酸酶酶解后同时提取，并通过LC/MS/MS在7 min内使用正离子模式下的电喷雾电离和多反应监测作为采集模式进行分析。所有化合物的保留时间（CV% < 2%）和相对丰度（CV% < 10%）均显示出良好的重现性。抗雌激素、糖皮质激素和类固醇的检测限在1-30 ng/mL范围内，兴奋剂的检测限在100-200 ng/mL范围内，因此满足世界反兴奋剂机构要求的最低性能限值。版权所有（c）2006约翰威利父子有限公司。
A fast liquid chromatographic/mass spectrometric (LC/MS/MS) screening method for the detection, in urine, of synthetic glucocorticoids, stimulants (formoterol, modafinil and mesocarb), anti-oestrogens (finasteride, exemestane, anastrozole, letrozole and formestane) and synthetic anabolic steroids (stanozolol, gestrinone and tetrahydrogestrinone) is described. All these drugs (and/or their urinary metabolites) can be simultaneously extracted by a single liquid/liquid extraction step, at alkaline pH. after enzymatic hydrolysis with beta-glucuronidase, and assayed in 7 min by LC/MS/MS using electrospray ionization in positive ion mode and multiple reaction monitoring as the acquisition mode. All compounds show good reproducibility of both the retention times (CV% < 2%) and the relative abundances (CV% < 10%). The limits of detection for the anti-oestrogens, glucocorticoids and steroids are in the range of 1-30 ng/mL, and for the stimulants are in the range of 100-200 ng/mL, thus satisfying the minimum required performance limits of the World Anti-Doping Agency. Copyright (c) 2006 John Wiley & Sons, Ltd.