Aurantimonas coralicida gen. nov., sp nov., the causative agent of white plague type II on Caribbean scleractinian corals
Aurantimonas coralicida gen. nov., sp nov., the causative agent of white plague type II on Caribbean scleractinian corals
复制标题
DOI：
10.1099/ijs.0.02359-0
复制
发表时间：
2003-07-01
影响因子：
2.8
通讯作者：
Richardson, LL
中科院分区：
文献类型：
--
作者：
Denner, EBM;Smith, GW;Richardson, LL
先前从硬壳珊瑚Dichocoenia stokesi(俗称椭圆形星状珊瑚)的患病群体中分离出的一株细菌进行了详细的多相分类鉴定。该菌株命名为WP1(T)，嗜盐，严格需氧，经长时间培养后形成金橙色菌落。WP1T细胞革兰氏阴性，呈杆状，呈典型的分枝杆状。从化学分类上看，WP1(T)的特征是以Q-10为主要呼吸脂醌，以均一高精胺为主要成分。细胞脂肪酸组成以C(18：1)omega7c为主，其次是C-19：0环omega8c和C-16：0。其他少量存在的脂肪酸是C-17：0，C-18：0，C(16：1)omega7c，C(20：1)omega7c和C-18：12-OH。主要极性脂类为磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱。存在少量的二磷脂酰甘油、磷脂酰单甲基乙醇胺和磷脂酰二甲基乙醇胺。基因组DNA中G+C含量为66.3mol%。16S rRNA基因序列的系统发育分析表明，WP1(T)代表了‘根瘤目’中的一个独立亚系。新的血统属于包括根瘤科、巴豆科、布鲁氏科和叶下科的科群，在这个科群中没有特别的亲戚。16S rRNA基因序列与该群内所有已建立的类群的相似性不超过92.0%(与地中海中根瘤菌)。为了适应这种新出现的珊瑚病原体，建议创建一个新的属和种，珊瑚金藻Aurantimonas coralicida gen。Nov.，sp.11月(模式株Wp1(T)=CIP 107386(T)=Dsm 14790(T))。
A bacterium previously isolated from a diseased colony of the scleractinian coral Dichocoenia stokesi (common name elliptical star coral) was subjected to a detailed polyphasic taxonomic characterization. The isolate, designated WP1(T), was halophilic and strictly aerobic and formed golden-orange-pigmented colonies after prolonged incubation. Cells of WP1T were Gram-negative, rod-shaped and showed a characteristic branching rod morphology. Chemotaxonomically, WP1(T) was characterized by having Q-10 as the major respiratory lipoquinone and sym-homospermidine as the main component of the cellular polyamine content. The predominant constituent in the cellular fatty acid profile was C(18:1)omega7c, along with C-19:0 cyclo omega8c and C-16:0. Other fatty acids present in smaller amounts were C-17:0, C-18:0, C(16:1)omega7c, C(20:1)omega7c and C-18:1 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. Minor amounts of diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidyldimethylethanolamine were present. The G+C content of the genomic DNA was 66.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that WP1(T) represents a separate subline of descent within the order 'Rhizobiales' of the 'Alphaproteobacteria'. The new line of descent falls within the group of families that includes the Rhizobiaceae, Bartonellaceae, Brucellaceae and 'Phyllobacteriaceae', with no particular relative within this group. The 16S rRNA gene sequence similarity to all established taxa within this group was not higher than 92.0% (to Mesorhizobium mediterraneum). To accommodate this emerging coral pathogen, the creation of a new genus and species is proposed, Aurantimonas coralicida gen. nov., sp. nov. (type strain WP1(T) = CIP 107386(T) = DSM 14790(T)).