Fractalkine (FRK) levels in amniotic fluid and its production during pregnancy
Fractalkine (FRK) levels in amniotic fluid and its production during pregnancy
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DOI：
10.1093/molehr/gag009
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发表时间：
2003-02-01
影响因子：
4
通讯作者：
Murata, Y
中科院分区：
文献类型：
--
作者：
Shimoya, K;Zhang, Q;Murata, Y
Fractalkine是一种新的CX 3C趋化因子，对T细胞、单核细胞和自然杀伤（NK）细胞具有化学引诱活性。Western blot分析显示，在第二和第三孕期的羊水和羊膜中检测到Fractalkine蛋白为95 kDa条带。使用抗fractalkine多克隆抗体的免疫组化显示，在第二和第三孕期的羊膜和滋养层上皮细胞的阳性染色。新生儿尿液也含有可检测量的fractalkine。RT-PCR检测羊膜中Fractalkine mRNA转录本。为了确定羊水和羊膜中是否存在Fractalkine受体（CX（3）CR 1）阳性细胞，我们使用CX（3）CR 1的特异性引物进行RT-PCR。CX（3）CR 1阳性细胞迁移到羊水和羊膜中。目前的研究结果表明，羊水中发现的fractalkine可能有助于怀孕期间的免疫防御机制。
Fractalkine is a new CX3C chemokine that has chemoattractant activity for T cells, monocytes and natural killer (NK) cells. Western blot analysis revealed that fractalkine protein was detected as a 95 kDa band in both the amniotic fluid and the amnion during the second and third trimesters. Immunohistochemistry using an anti-fractalkine polyclonal antibody revealed positive staining of epithelial cells in amnion and trophoblasts in both the second and third trimesters. Neonatal urine also contained detectable amounts of fractalkine. RT-PCR detected fractalkine mRNA transcripts in the amnion. To determine whether fractalkine receptor (CX(3)CR1)-positive cells were present in amniotic fluid and amnion, we performed RT-PCR using specific primers for CX(3)CR1. CX(3)CR1-positive cells had migrated into the amniotic fluid and the amnion. The present findings suggest that fractalkine found in amniotic fluid may contribute to the immunodefence mechanism during pregnancy.