Zhongshania antarctica gen. nov., sp nov and Zhongshania guokunii sp nov., gammaproteobacteria respectively isolated from coastal attached (fast) ice and surface seawater of the Antarctic
Zhongshania antarctica gen. nov., sp nov and Zhongshania guokunii sp nov., gammaproteobacteria respectively isolated from coastal attached (fast) ice and surface seawater of the Antarctic
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DOI：
10.1099/ijs.0.026153-0
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发表时间：
2011-09-01
影响因子：
2.8
通讯作者：
Zhang, Yu-Zhong
中科院分区：
文献类型：
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作者：
Li, Hui-Juan;Zhang, Xi-Ying;Zhang, Yu-Zhong
从南极沿岸附着（固定）冰和表层海水样品中分离到两株革兰氏阴性、运动、好氧、过氧化氢酶和氧化酶阳性的杆状菌株，分别命名为菌株55 -23（T）和菌株66 -22（T）。两种菌株都能在4-35 ℃（最适30 ℃）和无NaCl条件下生长. 16 S rRNA基因序列分析表明，菌株G15 -23（T）和G16 -22（T）的亲缘关系较近（99.0%），属于γ-变形菌纲（Gammaproteobacteria），与海绵菌属（Spongiibacter）和Melitea属（Melitea）的亲缘关系最近（93.1-94.3%）。两种菌株中的主要细胞脂肪酸为C-17：1 omega 8 c、C-17：0和总特征3（C（16：1 omega）7 c和/或iso-C-15：0 2-OH）。菌株G15 -23（T）和G16 -22（T）基因组DNA G + C含量分别为51.5mol%和51.8mol%。菌株G15 -23（T）和G16 -22（T）的DNA相关性为50.9%。通过许多表型特性的差异，菌株E15 -23（T）和E16 -22（T）可以彼此区分，并与海绵杆菌属和蜜茶属物种区分。根据所提供的数据，菌株105 -23（T）和106 -22（T）代表γ-变形菌纲中一个新属的两个新种，nov.（模式种）和中山虫Zhongshania guokunii sp. nov.。模式菌株为天山钟山虫（Zhongshania anastritica），模式菌株为天山5 -23（T）（=KACC 14066（T）=CCTCC AB 209246（T）），模式菌株为天山6 -22（T）（=KACC 14532（T）=CCTCC AB 209247（T））。
Two Gram-negative, motile, aerobic, catalase- and oxidase-positive, rod-shaped strains, designated ZS5-23(T) and ZS6-22(T), were respectively isolated from Antarctic coastal attached (fast) ice and surface seawater samples. Both strains could grow at 4-35 degrees C (optimum 30 C) and in the absence of NaCl. Analyses of 16S rRNA gene sequences revealed that strains ZS5-23(T) and ZS6-22(T) were closely related to each other (99.0% sequence similarity) and belonged to the class Gammaproteobacteria, with their closest relatives being Spongiibacter and Melitea species (93.1-94.3% sequence similarity). The predominant cellular fatty acids in both strains were C-17 : 1 omega 8c, C-17 : 0 and summed feature 3 (C(16 : 1 omega)7c and/or iso-C-15 : 0 2-0H). Genomic DNA G + C contents of strains ZS5-23(T) and ZS6-22(T) were 51.5 and 51.8 mol%, respectively. The DNA DNA relatedness between strains ZS5-23(T) and ZS6-22(T) was 50.9%. Strains ZS5-23(T) and ZS6-22(T) could be differentiated from each other and from Spongiibacter and Melitea species by differences in a number of phenotypic properties. Based on the data presented, strains ZS5-23(T) and ZS6-22(T) represent two novel species in a new genus in the class Gammaproteobacteria, for which the names Zhongshania antarctica gen. nov., sp. nov. (the type species) and Zhongshania guokunii sp. nov. are proposed. The type strain of Zhongshania antarctica is ZS5-23(T) (=KACC 14066(T) =CCTCC AB 209246(T)) and that of Zhongshania guokunii is ZS6-22(T) (=KACC 14532(T) =CCTCC AB 209247(T)).