Functional interaction of varicella zoster virus gene 62 protein with the DNA sequence bound by herpes simplex virus ICP4 protein.
Functional interaction of varicella zoster virus gene 62 protein with the DNA sequence bound by herpes simplex virus ICP4 protein.
复制标题
水痘带状疱疹病毒基因 62 蛋白与单纯疱疹病毒 ICP4 蛋白结合的 DNA 序列的功能相互作用。
DOI：
10.1006/viro.1993.1432
复制
发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
Wydoski,SG
中科院分区：
文献类型：
--
作者：
Betz,JL;Wydoski,SG
水痘带状疱疹病毒基因62蛋白[定义为开放阅读框（ORF） 62的产物]与其1型单纯疱疹病毒同源物ICP4 （α4, Vmw175）一样，从早期HSV-1糖蛋白D启动子反式激活基因表达。在瞬时共转染实验中，启动子中存在克隆的α4结合位点，增加了VZV蛋白对gD基因表达的反式激活。细菌提取物含有与ICP4 DNA结合域（2区）同源的VZV ORF62 230个氨基酸结构域，与α4BS DNA序列形成特异性配合物。这些结果表明，VZV ORF62蛋白与ICP4一样，以序列特异性的方式结合DNA，这种结合可能有助于其激活基因表达的机制。
Varicella zoster virus gene 62 protein [defined as the product of open reading frame (ORF) 62], like its herpes simplex virus type-1 homolog ICP4 (α4, Vmw175),trans-activates gene expression from the early HSV-1 glycoprotein D promoter. In transient co-transfection assays,trans-activation of gD gene expression by the VZV protein was increased by the presence of cloned α4 binding sites in the promoter. Bacterial extracts containing the 230-amino acid domain of VZV ORF62 homologous to the DNA binding domain of ICP4 (region 2) formed specific complexes with the α4BS DNA sequence. These results indicate that VZV ORF62 protein, like ICP4, binds DNA in a sequence-specific fashion, and the binding may contribute to its mechanism of activation of gene expression.