Dynamics of Biofilm Regrowth in Drinking Water Distribution Systems.
Dynamics of Biofilm Regrowth in Drinking Water Distribution Systems.
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DOI：
10.1128/aem.00109-16
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发表时间：
2016-07-15
影响因子：
4.4
通讯作者：
Boxall J
中科院分区：
文献类型：
--
作者：
Douterelo I;Husband S;Loza V;Boxall J
配水系统中的大部分生物质是以附着生物膜的形式存在。这是已知的饮用水处理后水质退化的核心，但这些高度异质性的社区存在的动态了解甚少。本文提供了有关此类动态的原始信息，研究结果展示了物质积累、季节性和影响因素的模式。本文介绍了在英国一个运行氯化系统上重复进行的为期1年的严格冲洗操作。进行了密集的监测和采样，包括时间序列浊度和使用16S rRNA Illumina MiSeq测序的详细微生物分析。结果表明，细菌动力学的影响，在供应的水和材料的差异仍然附着在管壁冲洗后。与细菌分布相关的主要因子是底泥、重金属和磷酸盐。再加上缺乏抑制生物膜的发展，由于余氯，这表明，限制无机营养物质，而不是有机碳，可能是一个可行的组成部分，在处理策略，以管理生物膜。该研究还表明，重复冲洗产生了有益的选择性压力，为冲洗成为一种可行的有利生物膜管理选择提供了另一个原因。这项工作促进了我们对饮用水分配系统中微生物过程的理解，并有助于为优化资产性能的战略提供信息。重要性这项研究提供了新的信息生物膜形成的动态真实的饮用水配水系统由不同的材料。供水系统中微生物过程的新知识可用于优化分配网络的性能，并确保消费者获得安全优质的饮用水。
The majority of biomass within water distribution systems is in the form of attached biofilm. This is known to be central to drinking water quality degradation following treatment, yet little understanding of the dynamics of these highly heterogeneous communities exists. This paper presents original information on such dynamics, with findings demonstrating patterns of material accumulation, seasonality, and influential factors. Rigorous flushing operations repeated over a 1-year period on an operational chlorinated system in the United Kingdom are presented here. Intensive monitoring and sampling were undertaken, including time-series turbidity and detailed microbial analysis using 16S rRNA Illumina MiSeq sequencing. The results show that bacterial dynamics were influenced by differences in the supplied water and by the material remaining attached to the pipe wall following flushing. Turbidity, metals, and phosphate were the main factors correlated with the distribution of bacteria in the samples. Coupled with the lack of inhibition of biofilm development due to residual chlorine, this suggests that limiting inorganic nutrients, rather than organic carbon, might be a viable component in treatment strategies to manage biofilms. The research also showed that repeat flushing exerted beneficial selective pressure, giving another reason for flushing being a viable advantageous biofilm management option. This work advances our understanding of microbiological processes in drinking water distribution systems and helps inform strategies to optimize asset performance. IMPORTANCE This research provides novel information regarding the dynamics of biofilm formation in real drinking water distribution systems made of different materials. This new knowledge on microbiological process in water supply systems can be used to optimize the performance of the distribution network and to guarantee safe and good-quality drinking water to consumers.