EXPRESSION OF CATHEPSIN-D IN PRIMARY AND METASTATIC HUMAN-MELANOMA AND DYSPLASTIC NEVI
EXPRESSION OF CATHEPSIN-D IN PRIMARY AND METASTATIC HUMAN-MELANOMA AND DYSPLASTIC NEVI
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DOI：
10.1111/1523-1747.ep12665371
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发表时间：
1995-03-01
影响因子：
6.5
通讯作者：
MORDOH, J
中科院分区：
文献类型：
--
作者：
PODHAJCER, OL;BOVER, L;MORDOH, J
High levels of cytosolic cathepsin D expression have been associated with poop prognosis in breast cancer node-negative patients, In this work, we provide evidence that three cell lines established from human metastatic melanomas-IIB-MEL-J, IIB-MEL-LES, and IIB-MEL-IAN-express high levels of procathepsin D mRNA, IIB-MEL-J cells secreted into the conditioned media about 30% of the newly synthesized protein, which was active at acidic pH, Melanoma tumors arising in nude mice after injection of the three different cell lines expressed high levels of procathepsin D mRNA, Moreover, 13 human metastatic melanomas expressed variable levels of procathepsin D mRNA, To study the possible association between cathepsin D expression and melanoma development, samples corresponding to 10 primary tumors, 11 metastatic melanomas, 10 dysplastic nevi, 27 nevocellular nevi, and normal melanocytes were studied by immunohistochemistry for cathepsin D-specific staining, We found that cathepsin D was expressed in all of the dysplastic nevi and primary and metastatic melanomas tested but in only 18% of nevocellular nevi (five of 27), whereas normal melanocytes showed no cathepsin D expression, The overall data indicate that cathepsin D is expressed at a high level by melanoma cells, and because of its expression in preneoplastic lesions, it may be associated with melanoma development.
