Electrical stimulation changes human mesenchymal stem cells orientation and cytoskeleton organization
Electrical stimulation changes human mesenchymal stem cells orientation and cytoskeleton organization
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DOI：
10.1166/jbt.2017.1631
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发表时间：
2017-09
影响因子：
0.1
通讯作者：
Sahba Mobini;Ülle-Linda Talts;Ruikang Xue;N. Cassidy;S. Cartmell
中科院分区：
文献类型：
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作者：
Sahba Mobini;Ülle-Linda Talts;Ruikang Xue;N. Cassidy;S. Cartmell
电作为一种物理刺激，最近正在成为组织工程的一种有吸引力的工具。在这项研究中，我们模拟了一个简化的电生物反应器提供的电场，使用有限元分析。另外，人骨髓间充质干细胞(HMSCs)在电生物反应器中培养，每天施加100 mV/mm的电场1h。在培养第1、2、3、7天用CellProfiler分析细胞形态、取向和细胞骨架的变化。在成骨分化的早期阶段，电刺激细胞与化学刺激细胞的细胞骨架结构也进行了比较。结果表明，第7天，hMSCs的取向和细胞骨架肌动蛋白细丝在阴极区附近发生垂直于电场的重组。这一发现和分析方法有望为进一步研究电场中细胞形态变化的机制提供一个框架。
Electricity, as a physical stimulus, is recently becoming an attractive tool for tissue engineering. In this study we simulated the electrical field delivered by a simplified electro-bioreactor, using finite element analysis. In addition, human mesenchymal stem cells (hMSCs) were cultured in an electro-bioreactor and an electrical field of 100 mV/mm for 1 hour per day was applied. The cell profile, orientation and cytoskeleton changes by CellProfiler was analysed at 1, 2, 3 and 7 days. The cytoskeleton texture of cells exposed to electrical stimulation was also compared with cells exposed to chemical stimulation during an early phase of osteogenic differentiation. Results showed that hMSCs orientation and cytoskeleton actin filaments reorganize perpendicular to the electrical field in the vicinity of the cathode area at day 7. This finding and analysis method has the potential to provide a framework for future studies of mechanism underlying the changes in cell profile in electrical fields.