CULTURED SYMPATHETIC NEURONS SYNTHESIZE AND RELEASE THE CYTOKINE INTERLEUKIN-1-BETA
CULTURED SYMPATHETIC NEURONS SYNTHESIZE AND RELEASE THE CYTOKINE INTERLEUKIN-1-BETA
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DOI：
10.1073/pnas.89.21.10440
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发表时间：
1992-11-01
影响因子：
11.1
通讯作者：
KESSLER, JA
中科院分区：
文献类型：
--
作者：
FREIDIN, M;BENNETT, MVL;KESSLER, JA
自主神经元有助于调节免疫反应，神经系统和免疫系统之间存在相互作用。这项研究旨在定义可能构成这种相互作用的一些分子机制。免疫印迹分析表明，培养的交感神经元合成并释放细胞因子白细胞介素1β（IL-1β）。此外，培养的交感神经元的RNA印迹分析表明，神经元含有编码IL-1β的mRNA。之前的研究表明，交感神经节的外植体培养物以及神经元与神经节非神经元细胞的分离共培养物合成 P 物质，而 P 物质及其 mRNA 的原位水平较低。白细胞介素 1 受体的拮抗剂显着抑制培养物中 P 物质的增加，表明内源性 IL-1β 至少部分介导了合成反应。由于纯神经元培养物不含 P 物质，并且神经元合成并释放 IL-1β，因此细胞因子的作用需要神经节非神经元细胞的存在。这些观察结果表明自主神经元通过合成和分泌至少两种已知的免疫调节剂（细胞因子 IL-1β 和神经肽物质 P）来影响免疫反应。
Autonomic neurons help to regulate immune responses, and there are reciprocal interactions between the nervous and immune systems. This study seeks to define some of the molecular mechanisms that may underlie such interactions. Immunoblot analysis indicated that cultured sympathetic neurons synthesize and release the cytokine interleukin 1beta (IL-1beta). In addition, RNA blot analysis of cultured sympathetic neurons demonstrated that the neurons contain mRNA encoding IL-1beta. It was previously shown that explant cultures of sympathetic ganglia and dissociated cocultures of neurons with ganglionic nonneuronal cells synthesize substance P, whereas in situ levels of substance P and its mRNA are low. An antagonist at the interleukin 1 receptor markedly depressed this increase in substance P in cultures, suggesting that endogenous IL-1beta mediates the synthetic response, at least in part. Because pure neuronal cultures do not contain substance P and neurons synthesize and release IL-1beta, the actions of the cytokine require the presence of ganglion nonneuronal cells. These observations suggest a role for autonomic neurons in influencing immune responses by synthesizing and secreting at least two known immunoregulators, the cytokine IL-1beta and the neuropeptide substance P.