Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja Group using RAPDs and nuclear SSRs
Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja Group using RAPDs and nuclear SSRs
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DOI：
10.1007/s00122-006-0399-7
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
Spooner, D. M.
中科院分区：
文献类型：
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作者：
Ghislain, M.;Andrade, D.;Spooner, D. M.
马铃薯Solanum tuberosum L.巴西马铃薯品种群由广泛生长在从委内瑞拉西部到玻利维亚中部的安第斯山脉的马铃薯地方品种组成，由于其优良的烹饪特性和开发现代品种的其他特性，形成了重要的育种群体。它们被区分为短日照适应，二倍体倍性（2n = 2x = 24），和缺乏块茎休眠。这种核简单序列重复（nSSR或微卫星）的研究补充了先前的随机扩增多态性DNA（RAPD）的研究，探索使用这些标记，形成一个核心集合的马铃薯品种群。像这个以前的RAPD研究，我们分析了128加入的乌藨子组使用核微卫星（nSSR）。128份材料中有26份对22个nSSR标记保持不变。nSSR数据揭示了25个意想不到的三倍体和四倍体材料。102份材料的染色体计数证实了这些nSSR结果，并突出了7个三倍体或四倍体。因此，这些nSSR标记（除了1个）在92%的情况下是二倍体马铃薯倍性的良好指标。nSSR和RAPD结果：（1）对于其余70份二倍体和nSSR变异的材料是高度不一致的，（2）显示了nSSR有效地发现栽培马铃薯中许多倍性变异的效用，（3）支持使用栽培组，（而不是一个物种）分类栽培马铃薯，（4）未能支持遗传距离和地理距离之间的关系，（5）质疑使用任何单一类型的分子标记来构建核心集合。
The Solanum tuberosum L. Phureja Group consists of potato landraces widely grown in the Andes from western Venezuela to central Bolivia, and forms an important breeding stock due to their excellent culinary properties and other traits for developing modern varieties. They have been distinguished by short-day adaptation, diploid ploidy (2n = 2x = 24), and lack of tuber dormancy. This nuclear simple sequence repeat (nSSR or microsatellite) study complements a prior random amplified polymorphic DNA (RAPD) study to explore the use of these markers to form a core collection of cultivar groups of potatoes. Like this prior RAPD study, we analyzed 128 accessions of the Phureja Group using nuclear microsatellites (nSSR). Twenty-six of the 128 accessions were invariant for 22 nSSR markers assayed. The nSSR data uncovered 25 unexpected triploid and tetraploid accessions. Chromosome counts of the 102 accessions confirmed these nSSR results and highlighted seven more triploids or tetraploids. Thus, these nSSR markers (except 1) are good indicators of ploidy for diploid potatoes in 92% of the cases. The nSSR and RAPD results: (1) were highly discordant for the remaining 70 accessions that were diploid and variable in nSSR, (2) show the utility of nSSRs to effectively uncover many ploidy variants in cultivated potato, (3) support the use of a cultivar-group (rather than a species) classification of cultivated potato, (4) fail to support a relationship between genetic distance and geographic distance, (5) question the use of any single type of molecular marker to construct core collections.