Tribolium castaneum immune defense genes are differentially expressed in response to Bacillus thuringiensis toxins sharing common receptor molecules and exhibiting disparate toxicity
Tribolium castaneum immune defense genes are differentially expressed in response to Bacillus thuringiensis toxins sharing common receptor molecules and exhibiting disparate toxicity
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DOI：
10.1016/j.dci.2015.02.005
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发表时间：
2015-06-01
影响因子：
2.9
通讯作者：
Rausell, Carolina
中科院分区：
文献类型：
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作者：
Contreras, Estefania;Benito-Jardon, Maria;Rausell, Carolina
在赤拟谷盗幼虫中，我们已经通过RNA干扰敲低证明了苏云金芽孢杆菌Cry 3Ba毒素受体钙粘蛋白样和钠溶质同向转运蛋白也是活性较低的Cry 3Aa毒素的功能性受体。对B的敏感性差异。苏云金杆菌的侵染可能不仅依赖于毒素与受体的相互作用，而且还依赖于宿主的防御机制。比较B处理后免疫相关基因Apo-III和两种抗菌肽Defensin 3和Defensin 2的表达。苏云金杆菌挑战。所有三个基因的上调后Cry 3Ba孢子晶体中毒，而只有防御素基因的表达诱导后Cry 3Aa孢子晶体处理，证明宿主免疫反应和幼虫之间的可能关联的易感性B。苏云金杆菌。我们评估了T.结果发现防御素3的肽段对人类病原菌大肠杆菌、金黄色葡萄球菌和白色念珠菌有效，其中S.金黄色葡萄球菌最敏感。(C)2015爱思唯尔有限公司版权所有。
In Tribolium castaneum larvae we have demonstrated by RNA interference knockdown that the Bacillus thuringiensis Cry3Ba toxin receptors Cadherin-like and Sodium solute symporter proteins are also functional receptors of the less active Cry3Aa toxin. Differences in susceptibility to B. thuringiensis infection might not only rely on toxin-receptor interaction but also on host defense mechanisms. We compared the expression of the immune related genes encoding Apolipophorin-Ill and two antimicrobial peptides, Defensin3 and Defensin2 after B. thuringiensis challenge. All three genes were up-regulated following Cry3Ba spore-crystal intoxication whereas only Defensins gene expression was induced upon Cry3Aa spore-crystal treatment, evidencing a possible association between host immune response and larval susceptibility to B. thuringiensis. We assessed the antimicrobial activity spectra of T. castaneum defensins peptide fragments and found that a peptide fragment of Defensin3 was effective against the human microbial pathogens, Escherichia coil, Staphylococcus aureus and Candida albicans, being S. aureus the most susceptible one. (C) 2015 Elsevier Ltd. All rights reserved.