Spermatozoal transcriptome profiling for bull sperm motility: a potential tool to evaluate semen quality
Spermatozoal transcriptome profiling for bull sperm motility: a potential tool to evaluate semen quality
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DOI：
10.1530/rep-08-0503
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发表时间：
2009-07-01
期刊：
影响因子：
3.8
通讯作者：
Boissonneault, Guylain
中科院分区：
文献类型：
--
作者：
Bissonnette, Nathalie;Lesque-Sergerie, Jean-Philippe;Boissonneault, Guylain
关于公牛的繁殖力，建立体外研究结果与田间肥力之间的联系需要一种多参数方法，该方法测量各种结构和动态功能的完整性，如运动特征等。精子的异质性RNA模式可用于基因组分析，以评估精子发生和精子的生育潜力，这主要是因为这种特殊分化细胞的转录组处于静态状态。在之前的一项研究中，我们确定了一些通过基于聚合酶链式反应的cDNA消减鉴定的精子转录本与不返回率相关，这是一种田间肥力指标。在本研究中，将微阵列技术与差异RNA转录本提取相结合。我们已经证明，在这些基因中，一些转录本也与从同一射精液中分离出来的一群精子细胞的运动状态有关。我们强调了一项系统的数据分析和验证计划，该计划对于确定这方面的重要成绩单很重要。通过这种方法，我们发现编码丝氨酸/苏氨酸睾丸特异蛋白激酶(TSSK6)和金属蛋白酶非编码RNA(ADAM5P)的转录本与高运动性状态相关(P
Regarding bull fertility, establishing an association between in vitro findings and field fertility requires a multi-parametric approach that measures the integrity of various structures and dynamic functions, such as motion characteristics, among others. The heterogeneous RNA pattern of spermatozoa could be used in genomic analysis for evaluating both spermatogenesis and fertility potential of semen, mainly because of the static status of the transcriptome of this particular differentiated cell. In a previous study, we determined that some spermatozoal transcripts identified by PCR-based cDNA subtraction are associated with non-return rate, a field fertility index. in the present study, the microarray technology was used in conjunction with differential RNA transcript extraction. We have shown that among these genes, some transcripts are also associated with the motility status of a population of sperm cells fractionated from the same ejaculate. We highlighted a systematic data analysis and validation scheme important for the identification of significant transcripts in this context. With such an approach, we found that transcripts encoding a serine/threonine testis-specific protein kinase (TSSK6) and a metalloproteinase non coding RNA (ADAM5P) are associated with high-motility status (P