REGULATION OF HOST RNA LEVELS DURING BACULOVIRUS INFECTION
REGULATION OF HOST RNA LEVELS DURING BACULOVIRUS INFECTION
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DOI：
10.1016/0042-6822(88)90522-3
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发表时间：
1988-10-01
期刊：
影响因子：
3.7
通讯作者：
MILLER, LK
中科院分区：
文献类型：
--
作者：
OOI, BG;MILLER, LK
在杆状病毒苜蓿银纹夜蛾核型多角体病毒（Autographa californica nuclear polyhedrosis virus，AcMNPV）感染允许宿主昆虫细胞系草地贪夜蛾（Spodoptera frugiperda）IPLB-SF-21的过程中，宿主肌动蛋白、组蛋白和热休克70（heat shock 70，hsp 70）RNA的水平显著降低。宿主RNA水平的降低主要发生在复制过程的窄窗口期间，从感染后约12至18小时（p.i），对应于病毒的细胞外形式出芽进入培养基的阶段。晚期病毒蛋白似乎需要这种减少，因为放线菌酮，细胞溶质蛋白质合成的抑制剂，和aphidicolin，宿主和病毒DNA聚合酶的抑制剂，抑制肌动蛋白和组蛋白RNA水平的减少。Ac DNA对应于S.分离、测序并鉴定了棉铃虫线粒体细胞色素c氧化酶亚基III（COIII）基因。观察到该基因的两个差异调节的线粒体转录本。这些转录本中较大的转录本的水平依赖于活跃的胞质蛋白质合成，在病毒感染期间以类似于核宿主基因的方式降低。较小的COIII转录物在感染后至少24小时内是稳定的。但这种RNA的水平最终在感染后48小时下降。
During infection of the permissive host insect cell line Spodoptera frugiperda IPLB-SF-21 by the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV), the levels of host actin, histone, and heat shock 70 (hsp 70) RNAs are reduced substantially. Reduction of the host RNA levels occurs primarily during a narrow window of the replication process, from approximately 12 to 18 hr postinfection (p.i), corresponding to the phase in which the extracellular form of the virus buds into the media. A late viral protein appears to be required for this reduction since cycloheximide, an inhibitor of cytosolic protein synthesis, and aphidicolin, an inhibitor of host and viral DNA polymerases, inhibit the reduction of actin and histone RNA levels. Ac DNA corresponding to the carboxyl half of the S. frugiperda mitochondrial cytochrome c oxidase subunit III (COIII) gene was isolated, sequenced, and characterized. Two differentially regulated mitochondrial transcripts of this gene are observed. The level of the larger of these transcripts, which is dependent on active cytosolic protein synthesis, is reduced during virus infection in a fashion similar to that of the nuclear host genes. The smaller COIII transcript is stable until at least 24 hr p.i. but the level of this RNA eventually declines by 48 hr p.i.