Standardizing GC-MS metabolomics
Standardizing GC-MS metabolomics
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DOI：
10.1016/j.jchromb.2008.04.049
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发表时间：
2008-08-15
影响因子：
3
通讯作者：
Klapa, Maria I.
中科院分区：
文献类型：
--
作者：
Kanani, Harin;Chrysanthopoulos, Panagiotis K.;Klapa, Maria I.
代谢组学是最新推出的“组学”分析平台，目前正处于开发阶段。为了使代谢组学广泛应用于生物和临床研究和实践，需要解决有关数据验证和再现性的问题。气相色谱-质谱（GC-MS）仍将是代谢组学实验室的组成部分。本文讨论了GC-MS代谢组学中偏差的来源，并提供了其发生和对最终结果影响的实验证据。当可用时，纠正或解释这些偏差的方法被提出，以实现定量GC-MS代谢组学系统方法的标准化。(C)2008 Elsevier B. V.保留所有权利。
Metabolomics being the most recently introduced "omic" analytical platform is currently at its development phase. For the metabolomics to be broadly deployed to biological and clinical research and practice, issues regarding data validation and reproducibility need to be resolved. Gas chromatography-mass spectrometry (GC-MS) will remain integral part of the metabolomics laboratory. In this paper, the sources of biases in GC-MS metabolomics are discussed and experimental evidence for their Occurrence and impact on the final results is provided. When available, methods to correct or account for these biases are presented towards the standardization of a systematic methodology for quantitative GC-MS metabolomics. (C) 2008 Elsevier B.V. All rights reserved.