G‐CSF: Its relationship to leukemia differentiation‐inducing activity and other hemopoietic regulators
G‐CSF: Its relationship to leukemia differentiation‐inducing activity and other hemopoietic regulators
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G-CSF：其与白血病分化诱导活性和其他造血调节因子的关系
DOI：
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发表时间：
1982
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影响因子：
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通讯作者：
M. Moore
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文献类型：
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作者：
M. Moore
小鼠骨髓单核细胞白血病WEHI-3B存在分化诱导型（D+）和非诱导型（D-）细胞系。这两种细胞系都产生一种CSF物质，专门刺激中性粒细胞集落的形成。这种G-CSF与肥大细胞生长因子共纯化，但可以与M-和GM-CSF分离。NZB骨髓对G-CSF刺激无反应。WEHI-3B D+细胞可被鼠和人毒素后血清中存在的因子诱导至终末粒细胞分化，该因子不同于WEHI-3B D+或D− CM中存在的G-CSF，因为后者几乎没有或没有白血病分化诱导活性。内毒素处理C. parvum致敏小鼠导致同时诱导具有对髓性白血病细胞的选择性作用的血清活性、血清分化诱导活性和白血病集落抑制活性。这些因素协同作用，阻止白血病干细胞自我更新。结果表明，多种诱导因子可能具有有效的和选择性的抗白血病活性。
The murine myelomonocytic leukemia WEHI‐3B exists as differentiation‐inducible (D+) and noninducible (D−) cell lines. Both lines produce a CSF species that stimulates exclusively the formation of neutrophil granulocyte colonies. This G‐CSF copurifies with a mast cell growth factor but can be separated from M‐ and GM‐CSF. NZB bone marrow is unresponsive to G‐CSF stimulation. WEHI‐3B D+ cells can be induced to terminal granulocyte differentiation by a factor present in murine and human postendotoxin serum that is different from G‐CSF present in WEHI‐3B D+ or D− CM since the latter has little or no leukemia differentiation‐inducing activity. Endotoxin treatment of C. parvum primed mice leads to simultaneous induction of serum activities with selective action on myeloid leukemic cells, a serum differentiation inducing activity and a leukemic colony inhibitory activity. These factors act synergistically to block leukemic stem cell self‐renewal. The results suggest that a variety of inducible factors may have potent and selective antileukemic activity.